本土丝状真菌黑曲霉USM F4在固体底物发酵下利用棕榈仁饼生产甘露聚糖酶

Syarifah Ab, I. Darah, I. Omar
{"title":"本土丝状真菌黑曲霉USM F4在固体底物发酵下利用棕榈仁饼生产甘露聚糖酶","authors":"Syarifah Ab, I. Darah, I. Omar","doi":"10.5580/2779","DOIUrl":null,"url":null,"abstract":"A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"32 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"20","resultStr":"{\"title\":\"Utilization Of Palm Kernel Cake For The Production Of Mannanase By An Indigenous Filamentous Fungus, Aspergillus Niger USM F4 Under Solid Substrate Fermentation\",\"authors\":\"Syarifah Ab, I. Darah, I. Omar\",\"doi\":\"10.5580/2779\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/2779\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/2779","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 20

摘要

本研究利用一种产甘露聚糖酶的本土真菌黑曲霉USM F4,该真菌是从当地油棕加工植物的腐烂棕榈仁饼(PKC)中分离出来的。在以PKC为底物的固体底物发酵(SSF)下,真菌培养产生了高产的细胞外甘露聚糖酶。结果表明,经过5 d的培养,真菌生长的胞外甘露聚糖酶活性最高为433.84 U/ g底物,葡萄糖胺活性最高为0.96 mg /g底物。优化培养条件和培养基组成为PKC 10 g,粒径0.5 mm,含水量80% (v/w),接种量为1 × 107孢子/ml,培养温度30℃,静态(不混合),糖蜜2% (w/w),硝酸铵4% (w/w)。优化后的甘露聚糖酶产量较优化培养条件和培养基组成前提高53.68%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Utilization Of Palm Kernel Cake For The Production Of Mannanase By An Indigenous Filamentous Fungus, Aspergillus Niger USM F4 Under Solid Substrate Fermentation
A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A Case Of Fatal Diphtheria In A Paediatric Patient Identification and Characterization of a Putative Chemotaxis Protein, CheY, from the Oral Pathogen Campylobacter rectus. Oro-Pharyngeal Carriage And Antimicrobial Susceptibility Of Streptococcus Pneumoniae From Healthy Children Isolation, Electron Microscopy And Physicochemical Characterization Of A Brucellaphage Against Brucella Abortus Vaccine Strain S19 Comparison Of Prevalence And Antimicrobial Sensitivity Of Salmonella typhimurium In Apparently Healthy Cattle And Goat In Sango-Ota, Nigeria
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1