本土丝状真菌黑曲霉USM F4在固体底物发酵下利用棕榈仁饼生产甘露聚糖酶

Syarifah Ab, I. Darah, I. Omar
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引用次数: 20

摘要

本研究利用一种产甘露聚糖酶的本土真菌黑曲霉USM F4,该真菌是从当地油棕加工植物的腐烂棕榈仁饼(PKC)中分离出来的。在以PKC为底物的固体底物发酵(SSF)下,真菌培养产生了高产的细胞外甘露聚糖酶。结果表明,经过5 d的培养,真菌生长的胞外甘露聚糖酶活性最高为433.84 U/ g底物,葡萄糖胺活性最高为0.96 mg /g底物。优化培养条件和培养基组成为PKC 10 g,粒径0.5 mm,含水量80% (v/w),接种量为1 × 107孢子/ml,培养温度30℃,静态(不混合),糖蜜2% (w/w),硝酸铵4% (w/w)。优化后的甘露聚糖酶产量较优化培养条件和培养基组成前提高53.68%。
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Utilization Of Palm Kernel Cake For The Production Of Mannanase By An Indigenous Filamentous Fungus, Aspergillus Niger USM F4 Under Solid Substrate Fermentation
A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.
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