{"title":"本土丝状真菌黑曲霉USM F4在固体底物发酵下利用棕榈仁饼生产甘露聚糖酶","authors":"Syarifah Ab, I. Darah, I. Omar","doi":"10.5580/2779","DOIUrl":null,"url":null,"abstract":"A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":"32 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"20","resultStr":"{\"title\":\"Utilization Of Palm Kernel Cake For The Production Of Mannanase By An Indigenous Filamentous Fungus, Aspergillus Niger USM F4 Under Solid Substrate Fermentation\",\"authors\":\"Syarifah Ab, I. Darah, I. Omar\",\"doi\":\"10.5580/2779\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/2779\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/2779","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Utilization Of Palm Kernel Cake For The Production Of Mannanase By An Indigenous Filamentous Fungus, Aspergillus Niger USM F4 Under Solid Substrate Fermentation
A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.