转基因水稻的检测:沙特阿拉伯市售转基因水稻Oryza sativa PCR检测的协同验证研究

M. Ibrahim, I. Alaraidh, A. Amid, A. Farouk, S. Bazaid, R. Greiner, Abdullah Alghunaim
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引用次数: 3

摘要

已经进行了一项合作试验研究,以验证在沙特阿拉伯销售的转基因水稻的提取方法和随后的PCR检测。检测在沙特阿拉伯使用实时聚合酶链反应进行,阳性样本在马来西亚的另一个实验室使用聚合酶链反应和琼脂糖凝胶可视化进行验证。对这些样本进行了检测,以确定是否存在NOS终结者。共检测了150个样本,其中三个样本检测结果呈阳性,并在马来西亚重新检测。沙特阿拉伯转基因水稻的存在支持了开发精确定量和定性方法的必要性,以便对沙特阿拉伯市场上的转基因产品进行常规分析和检测。随着转基因产品在沙特阿拉伯市场的发现,其他中东国家也有意或无意地进口转基因作物也就不足为奇了。
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Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.
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