利用从土壤中分离的纤维素分解真菌,酿酒酵母细胞将纤维素转化为可发酵糖以生产乙醇

R. Devi, S. Shankar
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引用次数: 3

摘要

采用标准方法从土壤中分离出具有纤维素酶活性的真菌。发现两株菌株产生较高数量的纤维素酶。这两种菌株在以羧甲基纤维素为底物的液体摇培养中生产纤维素酶。比较了两株菌株的滤纸活性和CMCase活性。两株菌株9 d培养滤液也表现出最高的活性。在以CMC为底物的培养基中,通过酿酒酵母细胞的糖化和发酵,检验了乙醇的产量。两株菌株在培养9 d时乙醇产量最高。因此,目前的工作是比较两种菌株的纤维素酶活性和两种菌株同时糖化和发酵的酒精产量。
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Bioconversion of cellulose into fermentable sugars by Saccharomyces cerevisiae cells for the production of ethanol using Cellulolytic fungi isolated from soil
The fungal species with cellulase activity was isolated from the soil using standard procedures. Two isolates were found to produce relatively high amount of cellulase. The production of cellulase by the two isolates was achieved in liquid shake culture containing Carboxymethyl cellulose as substrate. The filter paper activity and CMCase activity of the two isolates were also compared. The 9 day culture filtrate of the two isolates also exhibited highest activity. Ethanol production was checked in the media containing CMC as substrate after saccharification and fermentation by Saccharomyces cerevisiae cells. The ethanol yield was found to be maximum on the 9 day culture of both the isolates. Thus the current work deals with comparison of cellulase activity of the two isolates and alcohol production of two isolates by simultaneous saccharification and fermentation.
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