{"title":"利用从土壤中分离的纤维素分解真菌,酿酒酵母细胞将纤维素转化为可发酵糖以生产乙醇","authors":"R. Devi, S. Shankar","doi":"10.5580/1620","DOIUrl":null,"url":null,"abstract":"The fungal species with cellulase activity was isolated from the soil using standard procedures. Two isolates were found to produce relatively high amount of cellulase. The production of cellulase by the two isolates was achieved in liquid shake culture containing Carboxymethyl cellulose as substrate. The filter paper activity and CMCase activity of the two isolates were also compared. The 9 day culture filtrate of the two isolates also exhibited highest activity. Ethanol production was checked in the media containing CMC as substrate after saccharification and fermentation by Saccharomyces cerevisiae cells. The ethanol yield was found to be maximum on the 9 day culture of both the isolates. Thus the current work deals with comparison of cellulase activity of the two isolates and alcohol production of two isolates by simultaneous saccharification and fermentation.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Bioconversion of cellulose into fermentable sugars by Saccharomyces cerevisiae cells for the production of ethanol using Cellulolytic fungi isolated from soil\",\"authors\":\"R. Devi, S. Shankar\",\"doi\":\"10.5580/1620\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The fungal species with cellulase activity was isolated from the soil using standard procedures. Two isolates were found to produce relatively high amount of cellulase. The production of cellulase by the two isolates was achieved in liquid shake culture containing Carboxymethyl cellulose as substrate. The filter paper activity and CMCase activity of the two isolates were also compared. The 9 day culture filtrate of the two isolates also exhibited highest activity. Ethanol production was checked in the media containing CMC as substrate after saccharification and fermentation by Saccharomyces cerevisiae cells. The ethanol yield was found to be maximum on the 9 day culture of both the isolates. Thus the current work deals with comparison of cellulase activity of the two isolates and alcohol production of two isolates by simultaneous saccharification and fermentation.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/1620\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1620","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Bioconversion of cellulose into fermentable sugars by Saccharomyces cerevisiae cells for the production of ethanol using Cellulolytic fungi isolated from soil
The fungal species with cellulase activity was isolated from the soil using standard procedures. Two isolates were found to produce relatively high amount of cellulase. The production of cellulase by the two isolates was achieved in liquid shake culture containing Carboxymethyl cellulose as substrate. The filter paper activity and CMCase activity of the two isolates were also compared. The 9 day culture filtrate of the two isolates also exhibited highest activity. Ethanol production was checked in the media containing CMC as substrate after saccharification and fermentation by Saccharomyces cerevisiae cells. The ethanol yield was found to be maximum on the 9 day culture of both the isolates. Thus the current work deals with comparison of cellulase activity of the two isolates and alcohol production of two isolates by simultaneous saccharification and fermentation.