SIMULTANEOUS DETERMINATION OF LEVAMISOLE AND OXYCLOZANIDE IN THE PHARMACEUTICAL PREPARATION BY CAPILLARY ELECTROPHORESIS

A sensitive, precise and accurate capillary electrophoresis method was developed for simultaneous determination of levamisole and oxyclozanide in pure sample and pharmaceutical preparation. Capillary electrophoresis was presented as a simple separation analytical method for the simultaneous analysis of the deliberated drugs within a shorter analytical run time. In this study, separation was achieved on fused silica capillary (30 cm - 50 µm internal diameter); background electrolyte solution consisted of phosphate buffer (40 mM, pH 7.9) and UV detection at 227 nm. The method showed to be linear (r 2 > 0.9998), precise (RSD <0.193%), accurate (recovery of 99.95% for levamisole and 100.12% for oxyclozanide), specific and robust. LOD and LOQ values were 0.099 µg mL -1 and 0.299 µg mL -1 respectively for levamisole and 0.075 µg mL -1 and 0.228 µg mL -1 respectively for oxyclozanide. The proposed method obtained well separation and had a perfect accuracy. The method was validated according to ICH guidelines and carried out for determination of the cited drugs in their pharmaceutical preparation.