酵母乙二醛酶I活性增强基因的克隆及其对酵母细胞大小的影响

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI:10.1016/0385-6380(88)90081-7

Kousaku Murata, Toshihiko Saikusa, Yoshiharu Inoue, Akira Kimura

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引用次数: 8

摘要

在载体质粒YEp13上克隆了一个增强酵母乙二醛酶I活性的基因，并将其作为一个3 kb的染色体DNA片段。与不含YEp13的对照细胞相比，含有该基因的酵母细胞中乙二醛酶I的活性增加了约5倍。携带该基因的酵母细胞也表现出对细胞毒性甲基乙二醛的抗性增强，细胞大小增大。结果表明，乙二醛酶I可能参与了甲基乙二醛的解毒和酵母细胞大小的调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular cloning of a gene enhancing yeast glyoxalase I activity and its effect on yeast cell size

A gene enhancing glyoxalase I activity of a yeast, Saccharomyces cerevisiae, was cloned on a vector plasmid YEp13 as a 3-kb chromosomal DNA fragment. The glyoxalase I activity in yeast cells with the gene increased approximately 5-fold compared with that of the control cells with or without YEp13. The yeast cells with the gene also showed an elevated resistance to cytotoxic methylglyoxal and had an increased cell size. The results suggested that the glyoxalase I might be involved in both the detoxification of methylglyoxal and in the regulation of yeast cell size.

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