HPLC/Chemiluminescence Detection of Methamphetamine and Amphetamine in Black and White Hair Samples

Black and white hair samples were obtained from black-, gray-(i. e., a mixture of black and white hair) and white-haired methamphetamine (MA) users, and MA and amphetamine (AP) were determined by HPLC using chemiluminescence detection (CL-HPLC). MA and AP were detected in black hair, and were found in the part of the hair that grew during the period of MA use. In the same subjects, MA concentrations were lower in white hair than in black hair and AP was not detected in white hair. This difference may be related to the affinity of MA and AP for melanin.