侧耳菇和香菇亲本、杂交和重组菌株菌丝生长的模拟

J. D. Valenzuela-Cobos, R. O. Rodríguez-Grimón, M. L. Jara-Bastidas, A. Grijalva-Endara, D. C. Zied, M. E. Garín-Aguilar, G. Valencia del Toro
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引用次数: 4

摘要

利用(麦芽提取物琼脂)MEA的菌丝生长直径计算了侧耳菇和香菇亲本菌株、杂交菌株和重组菌株的菌丝生长动力学。用菌丝生长量法测定小麦籽粒中菌丝的生长量。将参数调整为1个线性模型和3个非线性模型(Baranyi、Exponential、Logistic)来确定比生长速度(μmax)和滞后相位时间(λ)。除杂交菌株PD4xLC3外,侧耳菇和香菇的菌丝形态与重组菌株相似。采用4种数学模型对双核菌株在MEA上的菌丝动力学进行了分析,结果表明μmax在6.30 ~ 23.49 mm day - 1之间,滞后期在0.22 ~ 4.47 h之间;杂交菌株PO5xLC2和重组菌株PD1xPD4在MEA和小麦籽粒上的μmax和滞后期分别为0.08 ~ 11.41 cm3 day - 1和0.06 ~ 8.04 h。与包括亲本菌株在内的其他菌株相比,杂种菌株PO5xLC2和重组菌株PD1xPD4在MEA和小麦籽粒上的μmax和滞后期分别最高和最低。结果表明,侧耳菇和香菇的杂交和重组菌株在EMA和小麦籽粒上的生长动力学均高于亲本菌株。
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MODELING OF MYCELIAL GROWTH OF PARENTAL, HYBRID AND RECONSTITUTED STRAINS OF Pleurotus AND Lentinula
The kinetics of mycelial growth of parental strains, hybrid and reconstituted of Pleurotus and Lentinula was calculated using the mycelial growth diameter for (malt extract agar) MEA. For wheat grain was determinate using the mycelial growth volume. The parameters were adjusted to one Linear Model and 3 nonlinear models (Baranyi, Exponential, Logistic) to determine the specific growth speed (μmax) and lag phase time (λ). The hybrid and reconstituted strains of Pleurotus spp. and Lentinula edodes showed similar mycelial morphology with exception of the hybrid PD4xLC3. The mycelial kinetics of the dikaryotic strains on MEA using 4 mathematical models showed μmax between 6.30 and 23.49 mm day−1 and the lag phase ranged from 0.22 to 4.47 h; while on wheat grain presented μmax since 0.08 to 11.41 cm3 day−1 and lag duration between 0.06 and 8.04 h. The hybrid strain PO5xLC2 and the reconstituted strain of Pleurotus djamor PD1xPD4 showed the highest μmax and lowest lag phase on MEA and wheat grain respectively in comparison to the other strains include their parental strains. The results showed that the hybrid and reconstituted strains of Pleurotus and Lentinula presented high st my elial growth kinetics on EMA and on wheat grain in relation to their parental strains.
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