气相色谱-质谱联用-傅里叶变换-红外(FT-IR)技术筛选感染栎甲醇干瘿提取物

A. Hussein, G. Mohammed, M. Hadi, I. Hameed
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引用次数: 58

摘要

本研究采用甲醇提取法测定了槲皮的化学成分,并利用红外光谱技术报道了槲皮的主要功能成分。采用气相色谱-质谱(GC-MS)方法筛选植物化合物。从侵染菌甲醇提取物中鉴定出12种具有生物活性的植物化学成分。植物化学化合物的鉴定是基于峰面积、保留时间、分子量和分子式。气相色谱-质谱分析结果显示,该菌中含有顺式-对薄荷-1(7),8-二烯-2-醇,3-壬基酸,尿素,N,N′-双(2-羟乙基)-,3-三氟乙酰氧戊烷,蝶呤-6-羧酸,2,2-二氟七烷,y-谷甾醇,spirost -8-en-11- 1,3 -羟基-,(3s,5α,14s,20s,22s,25R)-, Curan,16,17-二氢-,(20xi.)-, 17- α。21s-28,30-双去甲藿烷,异胆酸乙酯,米尔霉素B,6,28-无氢-15-氯-25-异丙基-13-脱氢-5-。傅里叶变换红外(FTIR)分析证实了感染菌中存在烯烃、脂肪族氟化合物、硝基化合物、烷烃、氢键醇和酚类化合物。关键词:侵染栎;傅里叶变换-红外(FT-IR);气相色谱-质谱(GC-MS)分析;
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Phytochemical screening of methanolic dried galls extract of Quercus infectoria using gas chromatography-mass spectrometry (GC-MS) and Fourier transform-infrared (FT-IR)
The main objective of this study was to determine the phytochemical composition from the dried galls of Quercus infectoria, using methanolic extraction and report the main functional components by using infrared (IR) technique. The phytochemical compound screened by gas chromatography-mass spectrometry (GC-MS) method. Twelve bioactive phytochemical compounds were identified in the methanolic extract of Q. infectoria. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, and molecular formula. GC-MS analysis of Q. infectoria revealed the existence of the Cis-p-mentha -1(7),8-dien-2-ol, 3-Nonynoic acid, Urea, N,N´-bis(2-hydroxyethyl)-, 3-Trifluoroacetoxypentadecane, Pterin -6-carboxylic acid, 2,2-Difluoroheptacosanoic acid, y-Sitosterol, Spirost-8-en-11-one, 3-hydroxy-, (3s,5α,14s,20s,22s,25R)-, Curan,16,17-didehydro-,(20xi.)-, 17.alfa.21s-28,30-Bisnorhopane, Ethyl iso-allocholate, Milbemycin B,6,28-anhydro-15-chloro-25-isopropyl-13-dehydro-5-. The Fourier transform-infrared (FTIR) analysis of Q. infectoria proved the presence of alkenes, aliphatic fluoro compounds, nitro compounds, alkanes, hydrogen bonded alcohols, and phenols. Key words: Quercus infectoria, Fourier transform-infrared (FT-IR), gas chromatography-mass spectrometry (GC-MS) analysis, phytochemicals.
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