印度尼西亚龙目岛不同沿海地区桉树的抗氧化活性

E. Prasedya, N. W. R. Martyasari, Rizki Apriani, Sipti Mayshara, R. Fanani, H. Sunarpi
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引用次数: 8

摘要

绿色巨藻Ulva lactuca在印度尼西亚龙目岛的大部分沿海地区非常丰富。先前的研究表明,Ulva含有有价值的成分,如生物活性化合物、食物或生物燃料。在本研究中,我们评估了在龙目岛沿海地区不同地区发现的Ulva lactuca的抗氧化活性。这些海藻于2019年5月在Batu Layar (BL)、Tanjung Aan (TA)和Seriwe (SW)采集。采用DPPH(1,1-二苯基-2-苦基肼基)自由基清除法测定海藻乙醇提取物的抗氧化活性。用Folin-Ciocalteu试剂比色法测定总酚。虽然它们是同一物种,但所有样品都表现出不同的抗氧化活性。来自TA (ULA)和Batu Layar (ULB)的海藻对DPPH自由基的清除能力比来自Seriwe (ULS)的海藻具有更高的IC50,分别为522.23±43 μg/mL和534.76±14 μg/mL;IC50为682.23±23 μg/mL)。阳性对照抗坏血酸表现出较强的抗氧化活性(IC50为67±4.6 μg/mL)。ULS、ULB、ULA的总酚含量(TPC)分别为2.675±0.5 mgGAEg−1、5.13±0.8 mgGAEg−1、5.33±2.1 mgGAEg−1。黄酮类化合物含量分别为19.031±2.1 mgREg−1、25.315±2.6 mgREg−1和28。分别为053±1.58 mgREg−1。目前的数据表明,不同地点、不同环境条件下,海藻的生物活性会有所不同。在BL和TA中发现的条件较为极端的绿海藻Ulva lactuca可作为抗氧化剂的来源,可用于食品和制药工业。绿色巨藻Ulva lactuca在印度尼西亚龙目岛的大部分沿海地区非常丰富。先前的研究表明,Ulva含有有价值的成分,如生物活性化合物、食物或生物燃料。在本研究中,我们评估了在龙目岛沿海地区不同地区发现的Ulva lactuca的抗氧化活性。这些海藻于2019年5月在Batu Layar (BL)、Tanjung Aan (TA)和Seriwe (SW)采集。采用DPPH(1,1-二苯基-2-苦基肼基)自由基清除法测定海藻乙醇提取物的抗氧化活性。用Folin-Ciocalteu试剂比色法测定总酚。虽然它们是同一物种,但所有样品都表现出不同的抗氧化活性。来自TA (ULA)和Batu Layar (ULB)的海藻对DPPH自由基的清除能力比来自Seriwe (ULS)的海藻具有更高的IC50,分别为522.23±43 μg/mL和534.76±14 μg/mL;IC50为682.23±23 μg/mL)。然而,阳性对照Asco…
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Antioxidant activity of Ulva lactuca L. from different coastal locations of Lombok Island, Indonesia
Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Ascorbic acid demonstrated significantly stronger antioxidant activity with IC50 of 67 ± 4.6 μg/mL). Furthermore, the total phenolic contents (TPC) of ULS, ULB, ULA were 2.675 ± 0.5 mgGAEg−1, 5.13 ± 0.8 mgGAEg−1, 5.33 ± 2.1 mgGAEg−1 respectively. Flavonoid contents were 19.031 ± 2.1 mgREg−1, 25.315 ± 2.6 mgREg−1, and 28. 053 ± 1.58 mgREg−1 respectively. Current data shows that different locations with different environmental conditions could result in different biological activity of the seaweeds. Green seaweed Ulva lactuca found in BL and TA with more extreme conditions could be considered as source for antioxidants and could be utilized in food and pharmaceutical industry.Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Asco...
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