{"title":"用单克隆抗brdu抗体方法观察植物染色体复制带","authors":"K. Taniguchi, R. Tanaka","doi":"10.1266/JJG.66.485","DOIUrl":null,"url":null,"abstract":"In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"27 1","pages":"485-489"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method\",\"authors\":\"K. Taniguchi, R. Tanaka\",\"doi\":\"10.1266/JJG.66.485\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.\",\"PeriodicalId\":22578,\"journal\":{\"name\":\"The Japanese Journal of Genetics\",\"volume\":\"27 1\",\"pages\":\"485-489\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Japanese Journal of Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1266/JJG.66.485\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese Journal of Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/JJG.66.485","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method
In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n=16), Crepis capillaris (2n=6), Haplopappus gracilis (2n=4) and Chrysanthemum coronarium var. spatiosum (2n=18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.
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