青叶乙酸乙酯提取物的植物化学及抑菌活性评价

Bello, A.B, Suleiman, A., Idris, A.D
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引用次数: 0

摘要

目前药物研究的一个趋势是希望获得植物来源的新型抗菌素,作为一种替代疗法和抑制抗菌素耐药性的手段。本研究对青叶乙酸乙酯提取物的植物化学活性和抗菌活性进行了研究。采用乙酸乙酯冷萃取法提取青叶。采用标准方法对提取液进行了植物化学成分评价,并采用孔扩散技术对临床分离的部分细菌进行了抑菌效果评价。研究发现,植物提取物中含有一些植物化学物质、生物碱、皂苷、类黄酮、酚、蒽醌和类固醇,这些物质被认为是观察到抗菌作用的一个因素。分别在25 mg/mL、50 mg/mL、75mg/mL、100mg/mL条件下测定菌株对粗提物的敏感性。对大肠杆菌、伤寒沙门氏菌、肺炎克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌的生长抑制范围分别为9.66±1.54 ~ 13.00±0.00、10.00±1.00 ~ 11.00±1.00、9.33±0.57 ~ 11.00±1.00、14.00±0.00 ~ 17.33±1.52和9.33±0.57 ~ 10.66±1.52 mm。最低抑菌浓度分别为25mg/mL、12.5mg/mL、25mg/mL、25mg/mL、12.5mg/mL和25mg/mL,最低杀菌浓度分别为50mg/mL、25mg/mL、50mg/mL、25mg/mL和50mg/mL。观察到的抗菌作用被认为是由于在提取物中检测到生物碱、皂苷、黄酮类、酚、蒽醌和类固醇。结果显然证明了这种植物在治疗细菌感染中的传统使用,其病原体是本研究中使用的生物体。
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Phytochemistry and Antibacterial Assessment of Ethyl acetate Leaf Extract of Newbouldia laevis
A current trend in drug research is the desire to obtain novel antimicrobials with plant origins as an alternative therapy and a means to curb antimicrobial resistance. This study evaluated the phytochemistry and antibacterial activity of ethyl acetate leaf extract of Newbouldia laevis. The leaf of Newbouldia laevis was cold extracted with Ethyl acetate. The extract was evaluated for it phytochemical constituents using standard methods and antibacterial effect against some clinical bacterial isolates using well diffusion technique. The study revealed the presence of some phytochemicals, alkaloids, saponins, flavonoids, phenol, anthraquinones and steroids in the plant extract, which were believed to be a contributing factor to the observed antibacterial effects. The test isolates' susceptibility to the crude extract was measured at 25 mg/mL and 50 mg/mL, 75mg/mL and 100mg/mL. The growth inhibition of Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa ranges from 9.66±1.54 to 13.00±0.00, 10.00±1.00 to 11.00±1.00, 9.33±0.57 to 11.00±1.00, 14.00±0.00 to 17.33±1.52 and 9.33±0.57 to 10.66± 1.52 mm respectively. The Minimum Inhibitory Concentration was observed at 25mg/mL, 12.5mg/mL, 25mg/mL,12.5mg/mL and 25mg/mL against Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa respectively while the Minimum Bactericidal Concentrations was at 50mg/mL, 25mg/mL, 50mg/mL, 25mg/mL and 50mg/mL respectively. The observed antibacterial effects were believed to be due to the presence of alkaloids, saponins, flavonoids, phenol, anthraquinones and steroids detected in the extract. The results apparently justified the traditional use of this plant in treating bacterial infections whose causative agents are the organisms used in this study.
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