发酵脱脂芝麻抗氧化活性的评价

Eunji Kim, Seung-Wha Jo, Eun-Jung Yim, Kum-Suk Kim, B. Choi, Nam-rye Lee, Do-Youn Jung
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引用次数: 0

摘要

本试验研究了15株枯草芽孢杆菌发酵的脱脂芝麻(DSS)提取物的总木质素含量和抗氧化活性。对发酵前后DSS的抗氧化活性进行了分析。BCH3678 (1613.8 mg/l)和BCH3572 (1599.5 mg/l)发酵的DSS提取物中木脂素总含量相对较高。根据酚类化合物和类黄酮含量及DPPH自由基清除率测定抗氧化活性;SRCM103716的总酚类化合物含量最高,为2803.3 mg GAE/g,总黄酮含量为1553.1 mg/g,与抗氧化活性有很强的相关性。SRCM103716在37℃发酵24 h后,DSS提取物对DPPH的清除率最高,为66.5%。发酵后的DSS提取物,无论菌株如何,都比未发酵的对照具有更高的抗氧化活性,这些结果表明这些提取物可能是生物活性化合物的潜在来源。
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An Evaluation of the Anti-oxidant Activity of Fermented Defatted Sesame Seeds
This study was conducted to investigate the total lignin content and anti-oxidant activity in extracts of defatted sesame seeds (DSS) fermented with 15 strains of Bacillus subtilis. The anti-oxidant activities of DSS were analyzed both before and after fermentation. The total lignan content of the DSS extracts fermented with BCH3678 (1,613.8 mg/l) and BCH3572 (1,599.5 mg/l) were relatively high compared to other strains. Anti-oxidant activity was determined according to phenolic compound and flavonoid content and DPPH radical scavenging rate; the highest total phenolic compound content was provided by the DSS with SRCM103716 at 2,803.3 mg GAE/g which returned total flavonoid content of 1,553.1 mg/g as strong correlation of its anti-oxidant activity. The DSS extract fermented by SRCM103716 at 37°C for 24 hr showed the highest DPPH scavenging rate at 66.5%. The fermented DSS extracts, regardless of strain, demonstrated higher anti-oxidative activity than the unfermented control, and these results suggest that such extracts could be useful as a potential source of bioactive compounds.
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