定向自组装抗体分子的蛋白质工程

M. Aizawa, K. Yun, T. Haruyama, Y. Yanagida, E. Kobatake
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引用次数: 4

摘要

针对自组装抗体(IgG)分子的定向蛋白质工程已经发展了两种类型。第一种方法是将半胱氨酸基团标记到与IgG的Fc部分具有特定亲和力的蛋白a上。半胱氨酸标记的蛋白A在金表面自组装,然后IgG自组装面向抗原识别位点进入溶液阶段。第二种方法是在单链抗体的一端用脂质连接,在另一端用六组氨酸连接。将脂质和组氨酸尾部单链抗体包埋在脂质体中,使抗原识别位点出现在外球上,通过组氨酸尾部的螯合将外球固定在ni处理的云母表面。单个脂质体通过AFM的轻叩模式清晰成像。
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Protein engineering for self-assembling antibody molecules in an oriented manner

Two types of protein engineering have been developed for self-assembling antibody (IgG) molecules in an oriented manner. The first is to tag a cysteine group to Protein A which has a specific affinity to the Fc part of IgG. The cysteine-tagged Protein A was self-assembled on the gold surface, which was followed by self-assembling of IgG to face the antigen recognition sites to the solution phase. The second is concerned with tailing a single chain antibody by lipid at the one terminal and the hexahistidinyl group at the other. The lipid and histidine-tailed single chain antibody was embedded in a liposome to make the antigen recognition site appear on the outsphere, which was immobilized on the Ni-treated mica surface through chelating of the histidine tail. The individual liposome was clearly imaged by a tapping mode of AFM.

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