担子菌MT-17.24在聚阴离子纤维素生物降解中的潜在应用

A. Zubchenko, E. Kozhevnikova, A. Barkov, Y. Topolyuk, A. Shnyreva, V. Vinokurov, L. Magadova
{"title":"担子菌MT-17.24在聚阴离子纤维素生物降解中的潜在应用","authors":"A. Zubchenko, E. Kozhevnikova, A. Barkov, Y. Topolyuk, A. Shnyreva, V. Vinokurov, L. Magadova","doi":"10.21285/2227-2925-2021-11-3-472-480","DOIUrl":null,"url":null,"abstract":"Abstract: Despite their efficiency, existing methods to dispose of drilling fluids used in the construction of oil and gas wells (chemical treatment of spent solutions, thermal method, thickening) are often expensive and unsustainable. Basidiomycota are natural xylotroph destructors that process lignocellulosic substrate – one of the most stable biopolymers in nature. Prospects for using enzyme preparations based on Basidiomycota as biodestructors of organic substances are evident due to the high efficiency and zero-waste production. The aim was to obtain an enzyme preparation based on the Trametes hirsute MT-17.24 Basidiomycota strain and evaluate its ability to biodegrade polyanionic cellulose, used as a viscosifier for drilling fluids in the construction and repair of oil and gas wells. Screening of cellulase activity of the following strains was carried out: Fomitopsis pinicola MT-5.21, Fomes fomentarius MT-4.05, Lactarius necator, Schizophyllum commune MT-33.01, Trametes versicolor It-1, Trametes hirsute MT-17.24, Trametes hirsuta MT-24.24. To obtain the enzyme preparation, the T. hirsuta MT-17.24 strain was selected, which demonstrated the highest coefficient of cellulase activity (10.9). A medium for solid-phase cultivation of this strain was selected. Enzymatic activity of the enzyme preparation was studied on a model drilling fluid. A 10-hour experiment showed that the use of a 1% enzyme preparation leads to a decrease in the plastic viscosity of the drilling fluid from 16 to 8 mPa·s. The research results demonstrate the efficiency of enzyme preparations based on Basidiomycota in the biodestruction of polyanionic cellulose.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Potential use of basidiomycota Trametes hirsuta MT-17.24 in biodegradation of polyanionic cellulose\",\"authors\":\"A. Zubchenko, E. Kozhevnikova, A. Barkov, Y. Topolyuk, A. Shnyreva, V. Vinokurov, L. Magadova\",\"doi\":\"10.21285/2227-2925-2021-11-3-472-480\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract: Despite their efficiency, existing methods to dispose of drilling fluids used in the construction of oil and gas wells (chemical treatment of spent solutions, thermal method, thickening) are often expensive and unsustainable. Basidiomycota are natural xylotroph destructors that process lignocellulosic substrate – one of the most stable biopolymers in nature. Prospects for using enzyme preparations based on Basidiomycota as biodestructors of organic substances are evident due to the high efficiency and zero-waste production. The aim was to obtain an enzyme preparation based on the Trametes hirsute MT-17.24 Basidiomycota strain and evaluate its ability to biodegrade polyanionic cellulose, used as a viscosifier for drilling fluids in the construction and repair of oil and gas wells. Screening of cellulase activity of the following strains was carried out: Fomitopsis pinicola MT-5.21, Fomes fomentarius MT-4.05, Lactarius necator, Schizophyllum commune MT-33.01, Trametes versicolor It-1, Trametes hirsute MT-17.24, Trametes hirsuta MT-24.24. To obtain the enzyme preparation, the T. hirsuta MT-17.24 strain was selected, which demonstrated the highest coefficient of cellulase activity (10.9). A medium for solid-phase cultivation of this strain was selected. Enzymatic activity of the enzyme preparation was studied on a model drilling fluid. A 10-hour experiment showed that the use of a 1% enzyme preparation leads to a decrease in the plastic viscosity of the drilling fluid from 16 to 8 mPa·s. The research results demonstrate the efficiency of enzyme preparations based on Basidiomycota in the biodestruction of polyanionic cellulose.\",\"PeriodicalId\":20601,\"journal\":{\"name\":\"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21285/2227-2925-2021-11-3-472-480\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21285/2227-2925-2021-11-3-472-480","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

摘要/ Abstract摘要:尽管现有的处理油气井施工中钻井液的方法(化学处理废液、热法、增稠法)效率很高,但往往成本昂贵且不可持续。担子菌是天然的木质营养破坏者,处理木质纤维素基质-自然界中最稳定的生物聚合物之一。以担子菌为原料的酶制剂具有高效、零废弃物的特点,具有广阔的应用前景。目的是获得一种基于Trametes hirsute MT-17.24担子菌的酶制备,并评估其生物降解聚阴离子纤维素的能力,聚阴离子纤维素是油气井建设和修复中钻井液的增粘剂。筛选了以下菌株的纤维素酶活性:pinicola真菌MT-5.21、formentarius真菌MT-4.05、necator乳酸菌、Schizophyllum commune MT-33.01、Trametes versicolor It-1、Trametes hirsute MT-17.24、Trametes hirsuta MT-24.24。为获得纤维素酶制剂,选择毛孢T. MT-17.24菌株,其纤维素酶活性系数最高(10.9)。选择了菌株固相培养的培养基。在模拟钻井液中研究了酶制剂的酶活性。10小时的实验表明,添加1%的酶制剂可使钻井液的塑性粘度从16 mPa·s降至8 mPa·s。研究结果验证了担子菌酶制剂对聚阴离子纤维素的生物降解效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Potential use of basidiomycota Trametes hirsuta MT-17.24 in biodegradation of polyanionic cellulose
Abstract: Despite their efficiency, existing methods to dispose of drilling fluids used in the construction of oil and gas wells (chemical treatment of spent solutions, thermal method, thickening) are often expensive and unsustainable. Basidiomycota are natural xylotroph destructors that process lignocellulosic substrate – one of the most stable biopolymers in nature. Prospects for using enzyme preparations based on Basidiomycota as biodestructors of organic substances are evident due to the high efficiency and zero-waste production. The aim was to obtain an enzyme preparation based on the Trametes hirsute MT-17.24 Basidiomycota strain and evaluate its ability to biodegrade polyanionic cellulose, used as a viscosifier for drilling fluids in the construction and repair of oil and gas wells. Screening of cellulase activity of the following strains was carried out: Fomitopsis pinicola MT-5.21, Fomes fomentarius MT-4.05, Lactarius necator, Schizophyllum commune MT-33.01, Trametes versicolor It-1, Trametes hirsute MT-17.24, Trametes hirsuta MT-24.24. To obtain the enzyme preparation, the T. hirsuta MT-17.24 strain was selected, which demonstrated the highest coefficient of cellulase activity (10.9). A medium for solid-phase cultivation of this strain was selected. Enzymatic activity of the enzyme preparation was studied on a model drilling fluid. A 10-hour experiment showed that the use of a 1% enzyme preparation leads to a decrease in the plastic viscosity of the drilling fluid from 16 to 8 mPa·s. The research results demonstrate the efficiency of enzyme preparations based on Basidiomycota in the biodestruction of polyanionic cellulose.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Biochemical composition of zoned apple varieties growing in different agrocenoses Novel proton-conducting materials based on a polyethylene terephthalate track-etched membrane modified with an N, P-containing ionic liquid Selection of herbaceous cellulose-containing raw materials for biotechnological processing Biochemical composition of ciders from various raw materials Growth characteristics of lactic acid-producing strains using glucose syrup as a carbon source
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1