Maria Petrocheilou, V. Samanidou, L. Kovatsi, M. Tsolaki, I. Papadoyannis
{"title":"建立了一种简便、直接的HPLC-DAD同时测定脑脊液、血清和尿液中加兰他明、多奈哌齐和利瓦斯汀含量的方法","authors":"Maria Petrocheilou, V. Samanidou, L. Kovatsi, M. Tsolaki, I. Papadoyannis","doi":"10.17145/JAB.17.010","DOIUrl":null,"url":null,"abstract":"Galantamine (GAL), donepezil (DON) and rivastigmine (RIV) are cholinesterase inhibitors administered to patients who suffer from Alzheimer’s disease (AD). We have currently developed and validated an HPLC-DAD method for the determination of GAL, DON, RIV in cerebrospinal fluid (CSF), blood serum and urine. The retention times of the drugs were 1.5, 2.2 and 3.1 min, respectively and the total time of analysis was 5 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to clinical CSF, blood serum and urine samples. The currently developed method is a reliable tool for monitoring CSF, serum and urine galantamine, donepezil and rivastigmine levels in patients under treatment with these drugs.","PeriodicalId":15014,"journal":{"name":"Journal of Applied Bioanalysis","volume":"118 1","pages":"59-69"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":"{\"title\":\"A simple and direct HPLC-DAD method for the simultaneous determination of galantamine, donepezil and rivastigmine in cerebrospinal fluid, blood serum and urine\",\"authors\":\"Maria Petrocheilou, V. Samanidou, L. Kovatsi, M. Tsolaki, I. Papadoyannis\",\"doi\":\"10.17145/JAB.17.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Galantamine (GAL), donepezil (DON) and rivastigmine (RIV) are cholinesterase inhibitors administered to patients who suffer from Alzheimer’s disease (AD). We have currently developed and validated an HPLC-DAD method for the determination of GAL, DON, RIV in cerebrospinal fluid (CSF), blood serum and urine. The retention times of the drugs were 1.5, 2.2 and 3.1 min, respectively and the total time of analysis was 5 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to clinical CSF, blood serum and urine samples. The currently developed method is a reliable tool for monitoring CSF, serum and urine galantamine, donepezil and rivastigmine levels in patients under treatment with these drugs.\",\"PeriodicalId\":15014,\"journal\":{\"name\":\"Journal of Applied Bioanalysis\",\"volume\":\"118 1\",\"pages\":\"59-69\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Bioanalysis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17145/JAB.17.010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Bioanalysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17145/JAB.17.010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A simple and direct HPLC-DAD method for the simultaneous determination of galantamine, donepezil and rivastigmine in cerebrospinal fluid, blood serum and urine
Galantamine (GAL), donepezil (DON) and rivastigmine (RIV) are cholinesterase inhibitors administered to patients who suffer from Alzheimer’s disease (AD). We have currently developed and validated an HPLC-DAD method for the determination of GAL, DON, RIV in cerebrospinal fluid (CSF), blood serum and urine. The retention times of the drugs were 1.5, 2.2 and 3.1 min, respectively and the total time of analysis was 5 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to clinical CSF, blood serum and urine samples. The currently developed method is a reliable tool for monitoring CSF, serum and urine galantamine, donepezil and rivastigmine levels in patients under treatment with these drugs.
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