马动物流行链球菌分离株产生物膜的评价

Bilal Osamah Mohammed Mohammed, U. S. Ucan
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引用次数: 1

摘要

目的:测定马链球菌动物流行病分离株的生物膜生产能力。材料与方法:采用刚果红琼脂法、结晶紫染色法和扫描电镜(SEM)三种不同的方法,对从sel uk大学兽医学院微生物学系实验室采集的41株马动物流行链球菌进行生物膜形成评价。结果:刚果红琼脂法所得结果与结晶紫法所得结果不相容。31株阳性菌株中有19株在刚果红琼脂上呈强阳性,其中30株在结晶紫染色法中被认为是强生膜菌株。证实了生物膜的生成,并利用扫描电镜对生物膜层进行了检测。结论:我国马科临床医生应认识到马链球菌分离株具有体外形成生物膜的能力
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The evaluation of biofilm production by Streptococcus equi zooepidemicus isolates
Aim: The purpose of this study is to measure biofim production ca - pabilities of Streptococcus equi zooepidemicus isolates. Materials and Methods: Forty one horse strains of Streptococcus equi zooepidemicus from the laboratory stock collection of Microbi - ology Department of the Veterinary Faculty, Selçuk University were evaluated by the biofilm formation using three different methods: Congo red agar, crystal violet staining method and scanning electron microscopy (SEM). Results: Incompatibility between the results obtained from Congo red agar and crystal violet staining methods was detected. Ninete - en out of 31 positive strains gave strong positive on Congo red agar while 30 of them were considered as strongly biofilm producing strains in crystal violet staining method. The biofilm production was confirmed and the biofilm layers were detected by using the SEM microscope. Conclusion: Equine clinicians in this Country should be aware of that Streptococcus equi zooepidemicus isolates are capable of for - ming biofilm in vitro
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