黑草甲醇种子提取物的植物化学筛选、LD50测定及亚慢性毒性研究

A. Muazu
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引用次数: 1

摘要

黑草长期以来被用作香料、食品防腐剂,在许多国家还被用作草药。本研究对黑草(Nigella sativa, ASENS)甲醇种子提取物进行了植物化学筛选、ld50评价和大鼠亚慢性研究。采用标准方法对植物化学成分进行定性和定量测定。用25只大鼠进行急性毒性(ld50,口服,大鼠)和亚慢性毒性试验。亚慢性毒性实验将12只大鼠分为4组,每组3只大鼠。ⅰ组为正常对照组,ⅱ组、ⅲ组、ⅳ组分别以10、100、1000mg/kg体重剂量口服ASENS,疗程4周。植物化学分析表明,不同浓度的黄酮类、单宁类、皂苷类、生物碱类和黄酮类化合物均以心苷类含量最高。急性毒性研究显示,在5000mg/kg剂量下,未出现中毒或死亡迹象;亚慢性毒性研究显示,与正常对照相比,II至IV组血清肝酶活性(AST和ALT)、血清总蛋白、尿素、K +和肌酐浓度显著降低(p<0.05), ALP活性和血清Na +、Cl -、HCO -浓度呈剂量依赖性显著升高(p<0.05)。因此,从生化指标上看,ASENS对大鼠肝肾功能指标均无毒性作用,且在给药剂量下是相对安全的。关键词:黑草;急性毒性;亚慢性毒性;DOI: 10.7176/FSQM/102-06出版日期:2020年11月30日
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Phytochemical Screening, LD50 Determination and Sub-Chronic Toxicity Studies of Methanol Seeds Extract of Nigella Sativa
Nigella Sativa has long been used as a spice, food preservatives and in many countries as herbal medicine. This study was carried out to screen the Phytochemical, evaluate the LD 50 and sub-chronic studies of methanol seeds extract of Nigella sativa (ASENS) in rats. Qualitative and quantitative phytochemicals were determined using standard methods. A total of twenty five rats were used for acute toxicity (LD 50 , oral, rat) and sub-chronic toxicity. For the sub-chronic toxicity twelve rats were divided into four groups of three rats each. Group I served as normal control, groups II, III, IV were administered orally with ASENS at doses of 10, 100 and 1000mg/kg body weight respectively for four weeks. Phytochemical analysis revealed the presence of tannins, saponins, alkaloids and flavonoids at different concentrations with cardiac glycosides having the highest. Acute toxicity study shows no sign of toxicity or mortality up to dose of 5000mg/kg while sub-chronic toxicity showed a significant decrease (p<0.05) in serum liver enzymes activities (AST and ALT), serum concentration of total protein, urea, K + , and creatinine with significant increase (p<0.05) in ALP activity and serum concentrations of Na + , Cl - , and HCO - in groups II to IV in dose dependent pattern compared to the normal control. Therefore, findings showed that there are no toxic effects of ASENS on rat ’ s liver and kidneys functions indices as evidence by biochemical parameters and also it’s relatively safe at administered doses. Keywords: Nigella sativa , acute toxicity, Sub-chronic toxicity and seed extract. DOI: 10.7176/FSQM/102-06 Publication date: November 30 th 2020
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