辣椒品种提取物对真菌菌株体外生长的影响

Koffi Affoué Carole, K. Clément, Kossonou Yao Kamelé, Koffi Ahua René, Koffi-Nevry Rose
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引用次数: 0

摘要

本研究旨在考察辣椒不同提取物(水、70%乙醇水和缩醛)对赤霉、青霉、镰刀菌和黄曲霉生长的影响。用稀释法测定菌株的抑菌活性、抑菌参数(MIC、MFC、IC50)和存活率。辣椒水提取物和70%乙醇提取物对赤霉、青霉和镰刀菌的MFC/MIC比值均为2。对于甜椒(soudanais, attie, doux和oiseau)品种,真菌菌株的这些值在1到2之间变化。大多数菌株的上述比值均小于4。这些提取物对选定的菌株有抑菌作用。所得到的曲线有一个下降的外观,或多或少陡坡取决于提取物。这些曲线用于获得霉菌50%存活率的抑制浓度(IC50)。IC50最小值(0.2 ~ 0.3 mg/mL)时活性最高。因此,辣椒提取物对真菌菌株的体外生长具有一定的剂量依赖性。
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Effect of Capsicum annuum L. and Capsicum frutescens L. Varieties Extracts on in vitro Growth of Fungal Strains
This study aims to test different extracts (water, 70% ethanolic-water and acetal) of Capsicum sp. on the growth of Alternaria sp., Penicillium sp., Fusarium sp. and Aspergillus flavus. The nature of the antifungal activity, the antifungal parameters (MIC, MFC, IC50) and the percentage of survival of the strains were determined with the dilution method. On Alternaria sp., Penicillium sp. and Fusarium sp. strains, values of MFC/MIC ratios of water and 70% ethanol extracts of Capsicum annuum antillais were equal to two. Concerning Capsicum frutescens (soudanais, attie, doux and oiseau) varieties, these values varied from 1 to 2 on the fungal strains. These ratios for most of the strains were lower than 4. These extracts exerted a fungistatic action on chosen strains. The curves obtained had a declining appearance with more or less steep slopes depending on the extracts. These curved were used to obtain the inhibitory concentrations for 50% survival (IC50) of the moulds. The highest activity was achieved at the lowest IC50 values which varied from 0.2 mg/mL to 0.3 mg/mL. Therefore, Capsicum extracts proved to be active on in vitro growth of the fungal strains studied in a dose-dependent manner.
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