鸭肠炎病毒UL26.5基因同义密码子使用分析

Yao Zhang, A. Cheng, Mingshu Wang, Dekang Zhu, R. Jia, Fei Liu, Xiaoyue Chen
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引用次数: 7

摘要

分析鸭肠炎病毒(DEV) UL26.5基因密码子使用偏向性,为了解DEV的进化和发病机制以及选择合适的宿主表达系统提高靶基因在体内和体外的表达提供依据。本研究对DEV和27种参比疱疹病毒UL26.5基因的同义密码子使用情况进行了研究。结果表明,在DEV的UL26.5基因密码子中,位于第三位的A和T的同义密码子被广泛使用,G + C组成约束是决定UL26.5基因密码子使用偏向的主要因素。系统发育分析表明,DEV在进化上更接近禽疱疹病毒,后者进一步聚类到甲疱疹病毒科。共有21个密码子与大肠杆菌和酵母菌、25个密码子与智人、26个密码子与腺病毒存在明显的使用差异。因此,如果对某些密码子进行优化,酵母和大肠杆菌表达系统可能适合于DEV UL26.5基因的表达。
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Analysis of Synonymous Codon Usage in the UL26.5 Gene of Duck Enteritis Virus
The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The results reveals that the synonymous codons with A and T at the third codon positon have widely usage in the codon of UL26.5 gene of DEV. G + C compositional constraint is the main factor that determines the codon usage bias in UL26.5 gene. The phylogentic analysis suggested that DEV was evolutionarily closer to fowl herpesviruses which further clustered into Alphaherpesvirinae. There were both 21 codons showing distinct usage differences between DEV with Escherichia coli and yeast, 25 between DEV and Homo sapiens, 26 between DEV and adenovirus. Therefore the yeast and E. coli expression system may be suitable for the expression of DEV UL26.5 gene if some codons could be optimized.
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