T-RFLP研究方法在亚临床酮症奶牛瘤胃微生物群研究中的应用

A. Yashin, Elizabeth Kasatkina, Aleksey Prusakov, Vladimir Ponamarev, Alexander Lunegov
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摘要

本文介绍了利用现代分子遗传学方法- t - rflp分析(末端限制性片段长度多态性)鉴定临床健康高产奶牛和亚临床酮症奶牛瘤胃内容物微生物群落的可能性的相关研究结果。研究方法是基于对微生物基因组变异中的保守区域的分析。结果表明,该方法对实验动物瘤胃内容物微生物的鉴定具有较高的效率。在高产亚临床酮症奶牛的瘤胃内容物中发现了大量细菌、古生菌、原生动物和厌氧真菌。获得的数据使我们能够显著地扩大高产奶量奶牛亚临床酮症发病机制的信息。在病牛的瘤胃内容物中存在条件致病性和致病性微生物群,表明违反了瘤胃消化,从而导致动物同时发生非传染性疾病。
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The T-RFLP research method in the study of rumen microbiota in dairy cows with subclinical ketosis
The article presents the results of researches related to the study of the possibility of using the modern molecular genetics method -T-RFLP-analysis (terminal restriction fragment length polymorphism) to identify the community of microorganisms in the contents of the rumen in clinically healthy highly productive cows and cows with subclinical ketosis. The research methodology is based on the analysis of the conservative regions in the microorganisms’ genome variability. The results showed the high efficiency of the method used for the identification of microorganisms in the rumen contents in the studied animals. A large community of bacteria, archaea, protozoa and anaerobic fungi is determined in the rumen contents from highly productive cows with subclinical ketosis. The data obtained allow us to significantly amplify the information on the pathogenesis of subclinical ketosis in cows with high milk productivity. The presence of conditionally pathogenic and pathogenic microflora in sick cows in the rumen contents indicates a violation of rumen digestion, which leads to the development of concomitant non-contagious diseases in animals.
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