生物防治剂与壳聚糖对谷子足腐病的协同防治作用

Poojarani Malagitti, N. Umashankar, Raveendra Hr, Benherlal Ps, S. Tulja
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摘要

手指谷子(Eleusine coracana L. Gaertn.)是卡纳塔克邦南部广泛种植的谷子作物,它受到各种真菌、细菌和病毒病原体的侵袭,其中由罗尔夫氏菌核菌引起的脚腐病是灌溉和雨养条件下农民的主要制约因素。为了解决这个问题,在实验室条件下采用了一种环保的方法,使用壳聚糖和生物防治剂,因为已知它们具有积极的先天防御机制和促进更高产量的附带效益。研究中选择了9种不同浓度的壳聚糖,其中0.15%、0.20%和0.25%的壳聚糖对病原菌的抑制作用最大。利用孔扩散技术评价了生物制剂哈茨木霉和荧光假单胞菌与壳聚糖对罗氏菌核菌的协同作用,结果表明,生物制剂对哈茨木霉和荧光假单胞菌具有良好的抑菌效果,具有一定的降病效果,壳聚糖对生物制剂无有害作用。
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Synergistic effect of biocontrol agents and chitosan on control of foot rot disease in finger millet
Finger Millet (Eleusine coracana L. Gaertn.) is a widely grown millet crop in Southern Karnataka, it is attacked by various fungal, bacterial and virus pathogens, among them foot rot disease caused by Sclerotium rolfsii is a leading constraint for the farmers under both irrigated and rainfed conditions. For the management of this problem an eco-friendly approach was taken up in laboratory condition using chitosan and biocontrol agents, as they are known to have spin-off benefit of active innate defence mechanisms and promoting higher yields. In the research, chitosan of nine different concentrations were selected among them 0.15%, 0.20% and 0.25% showed the maximum inhibition of the pathogen. Along with chitosan, bio-agents viz., Trichoderma harzianum and Pseudomonas fluorescens were evaluated against Sclerotium rolfsii for their synergistic effect by well diffusion technique and it exhibited a positive effect on the inhibition of the pathogen showing the effectiveness in reducing the disease and chitosan not showing deleterious effect on bio-agents.
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