大白菜悬浮培养对血根碱生长和积累的影响

Biochemie und Physiologie der Pflanzen Pub Date : 1992-04-01 DOI:10.1016/S0015-3796(11)80018-1

Franca Tomè , Maria Laura Colombo

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引用次数: 4

摘要

研究了从愈伤组织中提取的大蓟悬浮培养细胞的生长和异喹啉类生物碱的积累。液体批培养由粗细胞聚集体组成;继代培养后每14天，其鲜量增加3倍。发现细胞培养产生相对大量的异喹啉生物碱;血根碱作为主要生物碱积累并释放到培养基中。

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Characterization of a Suspension Culture of Chelidonium majus L. on Growth and Accumulation of Sanguinarine

Cell suspension cultures of C. majus L. — obtained from callus — were characterized on growth and on accumulation of isoquinoline alkaloids. The liquid batch cultures consisted of coarse cell aggregates; their fresh volume becames triple each 14 days after subcultures. Cell cultures were found to produce relatively large amounts of isoquinoline alkaloids; sanguinarine is accumulated as main alkaloid and also released into the medium.

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