火龙果皮提取物介导的绿色合成纳米银及其对辣椒炭疽病的抗真菌活性研究

IF 0.2 Q4 HORTICULTURE Journal of Horticultural Sciences Pub Date : 2023-06-30 DOI:10.24154/jhs.v18i1.2165
Shivakumar Gowda, S. Sriram
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引用次数: 0

摘要

由于植物提取物具有成本效益和生态友好的特性,它们已被用作还原和稳定剂来合成各种金属基纳米颗粒。本研究采用绿色环保的方法,以火龙果(Hylocereus spp.)果皮水提物为原料,在80℃的碱性条件下合成纳米银(Ag NPs)。通过各种分析和显微技术对银纳米粒子进行了表征。Ag纳米粒子的紫外可见光谱在400 ~ 410 nm之间有一个特征峰。透射电镜和扫描电镜研究证实了平均尺寸为7纳米的球形单分散颗粒。能量色散x射线光谱(EDX)证实了主元素中银和氯化银的存在。x射线粉末衍射(XRD)光谱显示了合成的银和氯化银纳米颗粒的结晶性。合成的纳米颗粒在体外分生孢子萌发和扩散板实验中均显示出对炭疽病菌孢子的潜在抗真菌活性。合成的NPs对辣椒炭疽病的防治具有潜在的抗真菌作用。
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Dragon fruit peel extract mediated green synthesis of silver nanoparticles and their antifungal activity against Colletotrichum truncatum causing anthracnose in chilli
Plant extracts have been used as reducing and stabilising agents to synthesise various metal-based nanoparticles due to their cost-effective and eco-friendly nature. In the present work, a green and environment-friendly method is adopted for synthesising silver nanoparticles (Ag NPs) using a biowaste of dragon fruit (Hylocereus spp.) peel aqueous extract at 80ºC in an alkaline condition. The Ag NPs were characterised through various analytical and microscopic techniques. The UV-Vis spectra of Ag NPs showed a characteristic peak between 400 - 410 nm. Transmission and scanning electron microscopic studies confirmed spherical monodispersed particles with an average size of 7 nm. Energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver and silver chloride among the principal elements. The X-ray powder diffraction (XRD) spectra showed the crystalline nature of synthesised silver and silver chloride nanoparticles. The synthesised nanoparticles showed potential antifungal activity against Colletotrichum truncatum spores in both in vitro conidial germination and spread plate assays. The efficacy of the synthesised NPs confirmed that these NPs could be used as potential antifungal agents against C. truncatum to control anthracnose in chilli.
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来源期刊
Journal of Horticultural Sciences
Journal of Horticultural Sciences Agricultural and Biological Sciences-Plant Science
CiteScore
0.30
自引率
0.00%
发文量
0
审稿时长
6 weeks
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