{"title":"高度并行微流控平台中罕见表观遗传癌症生物标志物的数字DNA序列分析","authors":"C. O’Keefe, Sixuan Li, Tza-Huei Wang","doi":"10.1109/TRANSDUCERS.2019.8808784","DOIUrl":null,"url":null,"abstract":"We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.","PeriodicalId":6672,"journal":{"name":"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)","volume":"27 1","pages":"2-5"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform\",\"authors\":\"C. O’Keefe, Sixuan Li, Tza-Huei Wang\",\"doi\":\"10.1109/TRANSDUCERS.2019.8808784\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.\",\"PeriodicalId\":6672,\"journal\":{\"name\":\"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)\",\"volume\":\"27 1\",\"pages\":\"2-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/TRANSDUCERS.2019.8808784\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/TRANSDUCERS.2019.8808784","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform
We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001% methylated/unmethylated.
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