D. Bordea, I. Oroian, R. Pop, A. Odagiu, Adrian Milä‚Șan
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引用次数: 0
摘要
胡萝卜Erwinia carotovora是马铃薯栽培的重要威胁,产生软腐病。防治软腐病的策略还考虑到使用非常规、环保产品的可能性,如大蒜提取物(Allium sativum L.),甚至富含硒的大蒜作为胡萝卜Erwinia carotovora的防治剂。本研究的目的是研究不同浓度大蒜和有机硒大蒜(Allium sativum L.)提取物对马铃薯胡萝卜欧文菌(Erwinia carotovora)的影响。以新鲜大蒜鳞茎和有机富硒大蒜鳞茎为原料,分别以100%的浓度制备水浸提液,分离aliquote部分,分别配制10%和20%的稀释液。采用圆盘扩散法测定大蒜水提物的抑菌活性。采用STATISTICA 7.0 v. soft软件进行基础统计,并检验实验变量间差异的显著性。20%水溶液对大蒜的抑制直径平均为12.28 mm, 10%水溶液对大蒜的抑制直径平均为11.95 mm。差异无统计学意义。20%富硒大蒜水溶液对胡萝卜欧文菌(Erwinia carotovora)的抑制率最高,达到87.71%。
Comparative Performances of Aqueous Extracts of Garlic and Selenium Enriched Garlic against Erwinia Cartovora
Erwinia carotovora is an important threat for potato cultures, producing the soft rot disease. The strategies of fight against soft rot disease also takes into consideration the possibility of using unconventional, environmentally friendly products, as extracts of garlic (Allium sativum L.), or even selenium enriched garlic as control agents for Erwinia carotovora. The aim of this study is to emphasize the effects of different concentrations of aqueous garlic and organic selenium enriched garlic (Allium sativum L.) extracts upon Erwinia carotovora bacteria isolated from potato. Aqueous extracts were prepared from fresh bulbs of Allium sativum L. and organic selenium enriched Allium sativum L. as 100% concentration, and aliquote parts were isolated in order to prepare 10%, and 20%, dilutions were prepared. The disc diffusion method was chosen for determining antibacterial activity of aqueous garlic extracts. The STATISTICA 7.0 v. soft was used for basic statistics, and testing the significance of differences between experimental variants. The 20% aqueous solution produces a diameter of inhibition was in average of 12.28 mm and 10% aqueous garlic an average diameter of 11.95 mm. The differences were statistically not significant. The most important performances were recorded for 20% aqueous solution of selenium enriched garlic that emphasized the highest activity against Erwinia carotovora (87.71% zone of inhibition).