杀菌剂对大豆耐药根瘤菌接种效率的影响

К. P. Kukol, N. Vorobey, P. Pukhtaievych, L. Rybachenko, R. Y. Yakymchuk
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引用次数: 2

摘要

目标。在播种前用Maxym XL、Standak Top和Fever拌种和防喷杀菌剂Akanto Plus影响下,评价大豆接种抗农药根瘤菌日本慢根瘤菌的效果。方法。生理、微生物、气相色谱、植被实验、统计结果。据报道,在以硫代康唑为基础的杀菌剂Fever的作用下,大豆生长季节根系上形成的根瘤数量和重量低于对照和其他试验变量。与Fever相比,Maxym XL和Standak Top对抗杀菌剂日本芽孢杆菌B78 Tn5突变体参与的豆科植物与根瘤菌共生的毒性作用较小。结果表明,与单纯接种相比,施用种子农药和接种根瘤菌可使水稻三叶期氮素同化水平降低10.9% ~ 41.1%。然而,在生长季节,杀菌剂对豆科植物与根瘤菌共生功能的负面影响减弱。结论。在模式植被试验条件下,同时使用Standak Top和Fever并进行灭菌,单株生产力分别下降了7.0%和14.1%。农药Maxym XL与抗农药日本白蝇B78转座突变体的复合作用可使产量最大提高27.2%。所获得的数据证实了杀菌剂和固氮微生物同时使用的方便性,以确保较高的经济和保护效果。
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EFFECT OF FUNGICIDES ON THE EFFICIENCY OF SOYBEAN INOCULATION WITH PESTICIDE-RESISTANT NODULE BACTERIA
Objective. Evaluate the efficiency of soybean inoculation with pesticide-resistant nodule bacteria Bradyrhizobium japonicum under the influence of pre-sowing seed dressing with Maxym XL,Standak Top and Fever and preventive spraying of plants with the fungicide Akanto Plus. Methods. Physiological, microbiological, gas chromatographic, vegetation experiment, statistical.Results. The number and weight of nodules formed on soybean roots during the growing seasonunder the action of the prothioconazole-based fungicide Fever were reported to be lower comparedwith the control and other variants of experiment. Pesticides Maxym XL and Standak Top had a lesspronounced toxic effect on the formation of legume-rhizobial symbiosis with the participation offungicide-resistant B. japonicum B78 Tn5 mutant compared to Fever. A decrease in the level of N2assimilation by 10.9–41.1 % in the three-leaf phase was found with the combined use of seed pesticides and nodule bacteria included in the study, relative to the variant with inoculation only. However, during the growing season, the negative effect of fungicidal preparations on the functioning oflegume-rhizobial symbiosis decreased. Conclusion. Under the conditions of the model vegetationexperiment with the concomitant use of Standak Top and Fever and bacterization, a decrease in individual plant productivity by 7.0 and 14.1 %, respectively, has been reported. The complex actionof the pesticide Maxym XL and inoculation with pesticide-resistant B. japonicum B78 transposonmutant, contributed to the maximum increase in yield by 27.2 %. The obtained data confirm the expediency of selection of fungicides and nitrogen-fixing microorganisms for concomitant use to ensure high economic and protective effects.
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