紫薯(Ipomoea batatas l)、黑米(Oryza sativa l)和黑糯米(Oryza sativa v. glutinosa)花青素提取物中花青素的鉴定

S. Sumartini
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摘要

源自不同商品的花青素具有不同的组成成分和花青素含量。这无疑会影响食品中总花青素的含量。本地项目如;紫薯、黑米和黑糯米的花青素结构成分和含量不同。研究目的是测定紫甘薯、黑米和黑糯米花青素提取物中总花青素的含量,并对花青素进行定性和定量鉴定。研究方法采用实验方法,采用UHPLC-PDA (Ultra high - Performance Liquid chromatography)仪器系统进行定性和定量鉴定分析。通过ph差测量的研究,紫甘薯中总花青素提取物为0.6 mg / g,远低于黑米(5.50 mg / g)和黑糯米(6.00 mg / g)。g材料)。通过对紫薯花青素提取物的鉴定,其主要成分为Delfinidin、Cyanidin和Malvidin,含量分别为0.12 mg、0.12 mg和1.20mg / 100g。同时,黑米和黑糯米中分别检出氰胺和麦芽糖苷,含量分别为0.73和13.17 mg / 100g和1.07 mg和17.6 mg / 100g。
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Identification of Anthocyanidins in Anthocyanin Extract of Purple Sweet Potato (Ipomoea batatas l), black rice (Oryza sativa l), and Black Glutinous rice (Oryza sativa v. glutinosa)
Anthocyanins originating from different commodities have different constituent components and anthocyanidin levels. This will undoubtedly affect the anthocyanin content of total in foodstuffs. Local items such as; Purple sweet potato, black rice, and black glutinous rice have different structural components and levels of anthocyanidins. The research objective was to measure the total anthocyanin content and identify anthocyanidins qualitatively and quantitatively from anthocyanin extracts of purple sweet potato, black rice, and black glutinous rice. The research method used was an experimental method , qualitative and quantitative identification analyses were carried out using the UHPLC-PDA (Ultra high Performance Liquid Chromatographic) instrument system. Based on the study with pH-difference measurements, the total anthocyanin extract in purple sweet potato is 0.6 mg / g of material, much lower than black rice (5.50 mg / g of material) and black glutinous rice (6.00 mg / g of material). g material). Based on the identification of the purple sweet potato anthocyanin extract, the main components of anthocyanidins were Delfinidin, Cyanidin, and Malvidin with concentrations of 0.12 mg, 0.12 mg, and 1.20mg / 100g of ingredients, respectively. Meanwhile, black rice and black glutinous rice were identified with cyanidine and malvidin with concentrations of 0.73 and 13.17 mg / 100g and 1.07 mg and 17.6 mg / 100g of ingredients.
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