亚硒酸盐对填充真菌菌丝形态测定参数的影响

Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić
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摘要

硒盐长期以来一直被认为对生物体有潜在的有益和有害的影响。它存在于环境中,很容易被植物和真菌吸收,从而进入食物链。我们研究了亚硒酸盐存在时的细胞生长动力学,亚硒酸盐被认为比硒酸盐具有更大的毒性。在体内显微镜下观察亚硒酸盐(1 mM)对真菌从激活孢子到指数生长末期的生长情况的影响。以黑藻为模型丝状真菌。Se+4处理最显著的效果是抑制菌丝生长,在控制条件下,Se+4处理组的菌丝在指数生长期结束时(200±50µm, n = 50 vs 900±100µm, n = 40)比对照组短4倍以上。硒+4的作用是抑制而不是简单的延迟生长,因为在培养第27 ~ 30小时,硒+4处理组菌丝长度没有显著变化。由于显微镜是在活培养细胞上进行的,观察到未受干扰的细胞质流动,证实在所有测量的时间点菌丝都是活的。Se+4处理也显著降低了30h孢子直径(p = 0.0365),菌丝直径无显著变化。
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THE INFLUENCE OF SELENITE ON FILLAMENTOUS FUNGI HYPHA MORPHOMETRY PARAMETERS
Selenium salts have been known for long time to have a potential for both beneficial and harmful effects on living organisms. It is present in the environment, where it can be readily assimilated by plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 mM) on the growth of fungi from the activated spores to the end of the exponential growth were measured on several hypha morphological parameters by microscopy in vivo. Phycomyces blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not significantly altered.
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