{"title":"A型肉毒杆菌中引起大鼠子宫收缩的物质","authors":"S. Sato, T. Kawata","doi":"10.3412/jsb.24.189","DOIUrl":null,"url":null,"abstract":"A substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"42 1","pages":"189-194"},"PeriodicalIF":0.0000,"publicationDate":"1969-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Substance in Clostridium botulinum type A causing uterine contraction in rats\",\"authors\":\"S. Sato, T. Kawata\",\"doi\":\"10.3412/jsb.24.189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.\",\"PeriodicalId\":14812,\"journal\":{\"name\":\"Japanese journal of bacteriology\",\"volume\":\"42 1\",\"pages\":\"189-194\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1969-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese journal of bacteriology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3412/jsb.24.189\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of bacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3412/jsb.24.189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Substance in Clostridium botulinum type A causing uterine contraction in rats
A substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.
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