{"title":"锂对庆大霉素诱导的离体大鼠肾脏n -乙酰氨基葡萄糖苷酶释放的抑制作用","authors":"M. Djamali, M. Shahrokhi","doi":"10.1080/107691897229766","DOIUrl":null,"url":null,"abstract":"N-Acetyl--d-glucosaminidase (NAG) is one of the sensitive hydrolytic lysosomal enzymes that is released after renal tubular damage. We have studied gentamicin-induced nephrotoxicity by determining the NAG release in a perfused rat kidney model. Gentamicin at 100 ug/ml caused a time-dependent increase in enzymuria. The effect of pretreatment with 5 or 10 mmol/kg of lithium chloride, administered subcutaneously 24 h prior to perfusion with gentamicin, was also studied through the measurement of NAG activity. Lithium at 5 mmol/kg absolutely inhibited NAG release; 10 mmol/kg lithium significantly diminished the release of enzyme. The inhibitory effect of 5 mmol/kg of lithium may be due to its interference with phosphoinositide cycle in renal tubular lysosomal membrane.","PeriodicalId":87425,"journal":{"name":"Toxic substance mechanisms","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"INHIBITION BY LITHIUM OF GENTAMICININDUCED RELEASE OF N-ACETYL--DGLUCOSAMINIDASE IN ISOLATED PERFUSED RAT KIDNEY\",\"authors\":\"M. Djamali, M. Shahrokhi\",\"doi\":\"10.1080/107691897229766\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"N-Acetyl--d-glucosaminidase (NAG) is one of the sensitive hydrolytic lysosomal enzymes that is released after renal tubular damage. We have studied gentamicin-induced nephrotoxicity by determining the NAG release in a perfused rat kidney model. Gentamicin at 100 ug/ml caused a time-dependent increase in enzymuria. The effect of pretreatment with 5 or 10 mmol/kg of lithium chloride, administered subcutaneously 24 h prior to perfusion with gentamicin, was also studied through the measurement of NAG activity. Lithium at 5 mmol/kg absolutely inhibited NAG release; 10 mmol/kg lithium significantly diminished the release of enzyme. The inhibitory effect of 5 mmol/kg of lithium may be due to its interference with phosphoinositide cycle in renal tubular lysosomal membrane.\",\"PeriodicalId\":87425,\"journal\":{\"name\":\"Toxic substance mechanisms\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxic substance mechanisms\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/107691897229766\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxic substance mechanisms","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/107691897229766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
n -乙酰-d-氨基葡萄糖酶(NAG)是肾小管损伤后释放的一种敏感的水解溶酶体酶。我们通过测定灌流大鼠肾模型中NAG的释放量来研究庆大霉素引起的肾毒性。100 ug/ml庆大霉素引起酶血症的时间依赖性增加。庆大霉素灌注前24 h皮下给予5或10 mmol/kg氯化锂预处理,并通过测定NAG活性来研究预处理效果。5 mmol/kg锂绝对抑制NAG释放;10 mmol/kg锂显著降低酶的释放。5 mmol/kg锂的抑制作用可能与干扰肾小管溶酶体膜内磷酸肌苷循环有关。
INHIBITION BY LITHIUM OF GENTAMICININDUCED RELEASE OF N-ACETYL--DGLUCOSAMINIDASE IN ISOLATED PERFUSED RAT KIDNEY
N-Acetyl--d-glucosaminidase (NAG) is one of the sensitive hydrolytic lysosomal enzymes that is released after renal tubular damage. We have studied gentamicin-induced nephrotoxicity by determining the NAG release in a perfused rat kidney model. Gentamicin at 100 ug/ml caused a time-dependent increase in enzymuria. The effect of pretreatment with 5 or 10 mmol/kg of lithium chloride, administered subcutaneously 24 h prior to perfusion with gentamicin, was also studied through the measurement of NAG activity. Lithium at 5 mmol/kg absolutely inhibited NAG release; 10 mmol/kg lithium significantly diminished the release of enzyme. The inhibitory effect of 5 mmol/kg of lithium may be due to its interference with phosphoinositide cycle in renal tubular lysosomal membrane.