大戟植物化学及体外抗癌研究的详细研究

S. M, Beebi Hazara M, D. P, S. M, A. S, S. Y
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引用次数: 0

摘要

健康是当今生活的主要问题。大多数疾病是由于不适当的生活方式,食用不适当的功能食品和依赖快餐,垃圾食品。由于这些食物,氧化发生,氧化导致细胞裂解,并导致癌症,像大戟这样的植物在我们的环境中很常见。它们也被用于管理不同的人类疾病。采用水提液对这些植物叶片的抗癌活性进行了研究。这些提取物要经过植物化学分析。体外方法对人卵巢癌细胞系Pa-1细胞具有明显的细胞毒性,其IC50浓度为57.08ug/ml(该化合物的浓度具有杀死50%活细胞的能力),孵育24h后。MTT法是一种用于测定细胞增殖和细胞毒性的比色法,其原理是将黄色水溶性四氮唑染料MTT还原为甲醛晶体。结果提示EHE具有较低的IC50值,可能对人卵巢癌(Pa-1)细胞具有较强的抗癌作用。
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A detailed study of phytochemical and invitro anticancer study on euphorbia heterophylla .l
Health is the main issue of today’s life. Most the diseases are due to improper life style, Taking improper functional foods and by depend on fast, junk foods. Due to these foods, oxidation takes place and oxidation causes lysis of cell and it causes cancer Plants such as Euphorbia heterophylla are commonly found in our surroundings. They are also used for the management of different human diseases. The anticancer activities of leaves from these plants were investigated using aqueous extracts. The extracts are subjected to phytochemical analysis. The invitro methods were used for significant cytotoxicity on Human ovarian cancer cell lines, viz., Pa-1 cells with the IC50 concentrations (The Concentration of the Compound have the capacity to kill 50% of Viable Cells) at 57.08ug/ml respectively after the incubation period of 24hrs. The MTT assay is a colorimetric assay used for the determination of cell proliferation and cytotoxicity, based on reduction of the yellow coloured water soluble tetrazolium dye MTT to formazan crystals. The obtained results suggesting us that the EHE may have effective anticancer potential against the Human ovarian cancer (Pa-1) cells due to its low IC50 value.
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