丛枝菌根真菌Glomus mosseae (Nicol & Gerd.)的分离与序列分析3-磷酸甘油酸激酶(PGK)基因启动子区

L. Harrier
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引用次数: 13

摘要

从噬菌体基因组文库中分离出了Glomus mosseae 3- phospholy cerate kinase (GmPGK)基因启动子,是从这些共生真菌中分离和分析的少数启动子元件之一。分析揭示了在其他真菌PGK基因的启动子区域发现的几个基序的存在。特别是,与S. er-evisiae和niverhizopus上游激活元件(UAS)片段同源的DNA序列。这些UAS序列在调节PGK基因碳源中的重要性是已知的,并简要讨论了GmPGK基因启动子中两个碳源调控的UAS序列的存在及其在AM真菌生物学中的作用。
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Isolation and Sequence Analysis of the Arbuscular Mycorrhizal Fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-Phosphoglycerate Kinase (PGK) Gene Promoter Region
The Glomus mosseae 3-phosphogly cerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promoter elements to be isolated and analysed from these symbiotic fungi. The analysis revealed the presence of several motifs which are found in the promoter region of other fungal PGK genes. In particular, DNA sequences homologous to segments of the S. cer-evisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulated UAS sequences in the GmPGK gene promoter and its role in the biology of AM fungi is discussed briefly.
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