苹果果皮粉理化特性、总酚含量及自由基清除活性研究

M. Khalid Saeed, N. Zahra, A. Saeed, Q. Syed
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摘要

苹果皮在许多水果工业中被视为废物,但它是营养物质和植物化学物质的重要来源,尤其是具有清除自由基能力的多酚。目的:研究苹果果皮粉的理化性质及其TPC和自由基清除活性。方法:对苹果皮粉末进行近似分析。测定了苹果皮粉中总多酚的含量。酚类化合物的总含量以没食子酸当量表示,即mg GAE/100g样品的提取物干重。结果:水分(7.65±0.88%)、灰分(2.50±0.35%)、脂肪(1.18±0.02%)、纤维(15.25±1.03%)、蛋白质(2.79±0.05%)、碳水化合物(71.0±2.30%)、能量(312±4.70 Kcal/100g)。苹果皮粉甲醇提取物中总酚含量为320±5.4 mg GAE/100g,水提取物中总酚含量为201±4.20 mg/100g。在20 ~ 100µg/ml浓度下,苹果皮粉甲醇提取物对自由基的清除能力为25.40±1.30 ~ 69.2±3.80%,水提取物为14.30±1.05 ~ 45.62±1.90%,BHT为17.8±1.15 ~ 51.62±2.15%。结论:两种苹果皮粉末提取物均具有较好的总多酚含量和较强的自由基清除能力。这些发现表明,苹果皮粉是一种天然抗氧化剂,可以作为几种氧化应激相关退行性疾病的预防性治疗方法。
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Physicochemical Characteristics, Total Phenolic Content and Free Radical Scavenging Activity of Apple (Malus Domestica) Peel Powder
Apple peel is considered as a waste product in many fruit industries but it is a noteworthy source of nutrients and phytochemicals, particularly polyphenols which have the ability to scavenge free radicals. Objectives: To study the physicochemical properties of apple (Malus domestica) peel powder, as well as its TPC and free radical scavenging activities. Methods: Proximate analysis of the apple peels powder was carried out. Total polyphenol content of apple peel powder was determined. The total content of phenolic compounds was expressed as gallic acid equivalent, i.e. mg GAE/100g of extract dry weight of sample. Results: The results of physicochemical characteristics moisture (7.65±0.88%), ash (2.50±0.35%), fat (1.18±0.02%), fiber (15.25±1.03%), protein (2.79±0.05%), carbohydrates (71.0±2.30%) and energy were 312±4.70 Kcal/100g. The total phenolic contents in the methanolic extract of apple peel powder were 320±5.4 mg GAE/100g while in H2O extract 201±4.20 mg/100g. The findings of the apple peel powder's methanolic extract's capacity to scavenge free radicals varied from 25.40±1.30—69.2±3.80%, while those of the water extract were 14.30±1.05—45.62±1.90%  and BHT were 17.8±1.15—51.62±2.15%  at concentration 20-100 µg/ml. Conclusions: The outcomes showed that both apple peel powder extracts had promising total polyphenols and have strong free radical scavenging activity. These findings suggest that the apple peel powder act as robust naturally occurring antioxidants and may be employed as a preventative therapy for several oxidative stress-related degenerative disorders.
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