合成[7-15N]-GTPs，利用核磁共振光谱分析 RNA 结构和动力学。

IF 1.7 Q3 ENGINEERING, GEOLOGICAL Geomechanics and Geoengineering-An International Journal Pub Date : 2022-01-01 Epub Date: 2022-02-26 DOI:10.1007/s00706-022-02892-1

Kehinde M Taiwo, Lukasz T Olenginski, Felix Nußbaumer, Hyeyeon Nam, Stefan Hilber, Christoph Kreutz, T Kwaku Dayie

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引用次数: 0

摘要

为利用核磁共振 (NMR) 光谱研究 RNA，已经开发出多种同位素标记策略。在此，我们报告了[7-15N]-鸟苷-5'-三磷酸的化学和酶法联合合成方法，该方法可通过基于 T7 RNA 聚合酶的体外转录掺入 RNA 中。我们展示了这些标签在探测两种具有重要生物学意义的 RNA 的结构和动力学方面的用途：在线版本包含补充材料，可查阅 10.1007/s00706-022-02892-1。

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Synthesis of [7-¹⁵N]-GTPs for RNA structure and dynamics by NMR spectroscopy.

Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-¹⁵N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s00706-022-02892-1.

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来源期刊

Geomechanics and Geoengineering-An International Journal ENGINEERING, GEOLOGICAL-

CiteScore

3.80

自引率

0.00%

发文量

期刊介绍： Geomechanics is concerned with the application of the principle of mechanics to earth-materials (namely geo-material). Geoengineering covers a wide range of engineering disciplines related to geo-materials, such as foundation engineering, slope engineering, tunnelling, rock engineering, engineering geology and geo-environmental engineering. Geomechanics and Geoengineering is a major publication channel for research in the areas of soil and rock mechanics, geotechnical and geological engineering, engineering geology, geo-environmental engineering and all geo-material related engineering and science disciplines. The Journal provides an international forum for the exchange of innovative ideas, especially between researchers in Asia and the rest of the world.