成熟对豚鼠远端结肠烟碱和毒蕈碱受体诱导的离子分泌的不同影响。

A. Powell, R. Reddix
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引用次数: 14

摘要

便秘的发病率随着年龄的增长而增加。这与调节肠道运动的肌神经元结构和功能的年龄相关变化有关;然而,粘膜下神经元的作用尚不清楚。本研究的目的是确定成熟对豚鼠结肠胆碱能受体诱导离子分泌的影响。对幼龄雄性豚鼠(3-4月龄)和成年雄性豚鼠(12-15月龄)肌肉剥离结肠段的短路电流(Isc)和组织电导的变化进行了监测。来自年轻豚鼠的31%的结肠段表现出持续的神经活动,这在成熟的动物中是不存在的。基线Isc仅在持续活动的年轻豚鼠组织中显著升高。所有组织的组织电导相似。电场刺激引起Isc的双相增加。在15 V/10 Hz时,只有峰1衰减,而在10 V/5Hz时,成熟豚鼠的两个峰均减弱。1,1,二甲基-4-苯基哌嗪(DMPP)诱导的离子分泌在成熟豚鼠中钝化。阿托品仅在年轻豚鼠中降低了1,1,二甲基-4-苯基哌嗪的反应。碳水化合物诱导的离子分泌在两个年龄组的组织中相似。综上所述,烟碱受体介导的胆碱能和非胆碱能分泌运动神经元的分泌均被减弱;然而,上皮毒蕈碱受体的活性在成熟过程中没有改变。
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Differential effects of maturation on nicotinic- and muscarinic receptor-induced ion secretion in guinea pig distal colon.
The incidence of constipation increases with age. This has been linked to age-related changes in the structure and function of myenteric neurons regulating intestinal motility; however, the role of submucous neurons is unknown. The aim of this study was to determine the effect of maturation on cholinergic receptor-induced ion secretion in guinea pig colon. Changes in the short-circuit current (Isc) and tissue conductance were monitored in muscle-stripped colonic segments from young (3-4-month-old) and mature (12-15-month-old) male guinea pigs. Thirty-one percent of colonic segments from young guinea pigs exhibited ongoing neural activity, which was absent in mature animals. Baseline Isc was significantly higher only in young guinea pig tissues with ongoing activity. Tissue conductance was similar in all tissues. Electrical field stimulation caused a biphasic increase in the Isc. At 15 V/10 Hz, only Peak 1 was attenuated, whereas both peaks were reduced in mature guinea pigs at 10 V/5Hz. 1,1, dimethyl-4-phenyl-piperazinium(DMPP)-induced ion secretion was blunted in mature guinea pigs. Atropine reduced the 1,1, dimethyl-4-phenyl-piperazinium response only in young guinea pigs. Carbachol-induced ion secretion was similar in tissues from both age groups. In conclusion, nicotinic receptor-induced secretion mediated by both cholinergic and noncholinergic secretomotor neurons was blunted; however, epithelial muscarinic receptor activity was unaltered during maturation.
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