{"title":"食品中耐粘菌素细菌的快速实用筛选方法","authors":"Yoshimasa Yamamoto","doi":"10.26717/bjstr.2021.40.006381","DOIUrl":null,"url":null,"abstract":"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food. Biomed J Sci & Res We developed a rapid and practical screening method for simultaneously detecting both Escherichia coli and bacteria harboring the mcr-1 colistin resistance gene using high-speed real-time polymerase chain reaction with specific TaqMan probes. The entire procedure, from sample processing to the final result, was performed within 1 h. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results indicated the potential of this method for the convenient and rapid screening of food items to detect contamination with mcr-1 –positive bacteria, which can be especially useful for on-site testing in developing countries.","PeriodicalId":9035,"journal":{"name":"Biomedical Journal of Scientific & Technical Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"\\\"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food\\\"\",\"authors\":\"Yoshimasa Yamamoto\",\"doi\":\"10.26717/bjstr.2021.40.006381\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food. Biomed J Sci & Res We developed a rapid and practical screening method for simultaneously detecting both Escherichia coli and bacteria harboring the mcr-1 colistin resistance gene using high-speed real-time polymerase chain reaction with specific TaqMan probes. The entire procedure, from sample processing to the final result, was performed within 1 h. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results indicated the potential of this method for the convenient and rapid screening of food items to detect contamination with mcr-1 –positive bacteria, which can be especially useful for on-site testing in developing countries.\",\"PeriodicalId\":9035,\"journal\":{\"name\":\"Biomedical Journal of Scientific & Technical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Journal of Scientific & Technical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26717/bjstr.2021.40.006381\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Journal of Scientific & Technical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26717/bjstr.2021.40.006381","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food"
Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food. Biomed J Sci & Res We developed a rapid and practical screening method for simultaneously detecting both Escherichia coli and bacteria harboring the mcr-1 colistin resistance gene using high-speed real-time polymerase chain reaction with specific TaqMan probes. The entire procedure, from sample processing to the final result, was performed within 1 h. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results indicated the potential of this method for the convenient and rapid screening of food items to detect contamination with mcr-1 –positive bacteria, which can be especially useful for on-site testing in developing countries.
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