通过BAC-FISH分析揭示了雀形目鸟类高度保守的微染色体组织

Q4 Agricultural and Biological Sciences Western Birds Pub Date : 2023-06-16 DOI:10.3390/birds4020020
M. S. de Souza, S. A. Barcellos, Victoria Tura, V. L. Bobrowski, A. D. Garnero, R. J. Gunski, D. Griffin, Rafael Kretschmer
{"title":"通过BAC-FISH分析揭示了雀形目鸟类高度保守的微染色体组织","authors":"M. S. de Souza, S. A. Barcellos, Victoria Tura, V. L. Bobrowski, A. D. Garnero, R. J. Gunski, D. Griffin, Rafael Kretschmer","doi":"10.3390/birds4020020","DOIUrl":null,"url":null,"abstract":"Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), and Double-collared Seedeater (Sporophila caerulescens)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.","PeriodicalId":52426,"journal":{"name":"Western Birds","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis\",\"authors\":\"M. S. de Souza, S. A. Barcellos, Victoria Tura, V. L. Bobrowski, A. D. Garnero, R. J. Gunski, D. Griffin, Rafael Kretschmer\",\"doi\":\"10.3390/birds4020020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), and Double-collared Seedeater (Sporophila caerulescens)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.\",\"PeriodicalId\":52426,\"journal\":{\"name\":\"Western Birds\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Western Birds\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/birds4020020\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Western Birds","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/birds4020020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

雀形目鸟类因其显著的多样性而被广泛认可,迄今已描述的物种超过5700种。像大多数鸟类一样,它们具有现代鸟类的核型特征,包括由几对大染色体和许多对微染色体组成的双峰核型。虽然典型的核型是2n = 80,但二倍体的数目在非典型情况下变化很大,从56到大约100条染色体不等。在本研究中,我们旨在利用细菌人工染色体,通过荧光原位杂交(bacfish)技术,利用鸡(Gallus Gallus)或斑胸草雀(Taeniopygia guttata)每条微染色体(GGA10-28,除GGA16外)的微染色体探针,了解该群体中4个物种核型组织结构的保守程度。通过对四种雀形目——纹捕蝇(Myiodynastes maculatus)、闪亮牛鹂(Molothrus bonariensis)、南方鹪鹩(Troglodytes aedon)和双领种子(Sporophila caerulescens)的染色体补体进行研究,发现了南方鹪鹩的一个新的染色体数目。通过FISH实验,我们能够观察到与鸟类共同祖先相同的微染色体组织模式。因此,我们提出了一个新的二倍体数目,并确认了微染色体组织的保存状态,这可能赋予该群体进化优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis
Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), and Double-collared Seedeater (Sporophila caerulescens)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Western Birds
Western Birds Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
0.60
自引率
0.00%
发文量
0
期刊最新文献
David F. DeSante’s Birds of Cabo San Lucas, Fall 1968: A Historic Account First Record of Tricolored Blackbirds in Idaho American Crow Cracks Open Bivalve via Automobile Second Prebasic Molt of a Black-headed Gull at Anchorage, Alaska Nesting Bald Eagle Population Numbers, Density, Territorial Resources, and Relationship to Human Development in Northern Colorado’s Front Range
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1