半精制卡拉胶减少脂多糖诱导的外周血单核细胞活性氧的产生和白细胞细胞膜的改变

Q3 Pharmacology, Toxicology and Pharmaceutics Journal of Pharmacy and Nutrition Sciences Pub Date : 2021-12-25 DOI:10.29169/1927-5951.2021.11.20
Y. Posokhov, A. Onishchenko, T. Chumachenko, N. Makieieva, Y. Kalashnyk-Vakulenko, H. Polikarpova, V. Novikova, V. Prokopyuk, O. Nakonechna, D. Chumachenko, Viktoriya Tkachenko, I. Meniailov, M. Tkachenko, A. Tkachenko
{"title":"半精制卡拉胶减少脂多糖诱导的外周血单核细胞活性氧的产生和白细胞细胞膜的改变","authors":"Y. Posokhov, A. Onishchenko, T. Chumachenko, N. Makieieva, Y. Kalashnyk-Vakulenko, H. Polikarpova, V. Novikova, V. Prokopyuk, O. Nakonechna, D. Chumachenko, Viktoriya Tkachenko, I. Meniailov, M. Tkachenko, A. Tkachenko","doi":"10.29169/1927-5951.2021.11.20","DOIUrl":null,"url":null,"abstract":"\nAim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes.\n\n\nMethods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml), LPS (1 µg/ml), E407a (10 mg/ml) + LPS (1 µg/ml), E407a (50 mg/ml) + LPS (1 µg/ml) and without those compounds (controls) for 2 h in RPMI 1640 medium enriched with 5% fetal bovine serum. ROS generation in PBMCs obtained from the incubated samples was estimated by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. The impact of E407a, LPS and their mixture on leukocyte cell membranes was evaluated spectrofluorimetrically using the fluorescent probe 2-(2¢-hydroxy-phenyl)-5-phenyl-1,3-oxazole.\n\n\nResults: Expectedly, incubation with LPS induced ROS generation in PBMCs and decreased the lipid order of cell membranes in leukocytes. E407a alone was found to alter neither ROS production in PBMCs, nor membrane lipid order in leukocytes. Semi-refined carrageenan partially reduced LPS-mediated ROS overproduction in PBMCs and cell membrane alterations in leukocytes.\nConclusion: E407a attenuates LPS-induced alterations of redox homeostasis in rat PBMCs and LPS-mediated modifications of cell membrane lipid order in leukocytes.\n","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"57 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Semi-Refined Carrageenan Attenuates Lipopolysaccharide-Induced Reactive Oxygen Species Production in Peripheral Blood Mononuclear Cells and Cell Membrane Alterations in Leukocytes\",\"authors\":\"Y. Posokhov, A. Onishchenko, T. Chumachenko, N. Makieieva, Y. Kalashnyk-Vakulenko, H. Polikarpova, V. Novikova, V. Prokopyuk, O. Nakonechna, D. Chumachenko, Viktoriya Tkachenko, I. Meniailov, M. Tkachenko, A. Tkachenko\",\"doi\":\"10.29169/1927-5951.2021.11.20\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\nAim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes.\\n\\n\\nMethods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml), LPS (1 µg/ml), E407a (10 mg/ml) + LPS (1 µg/ml), E407a (50 mg/ml) + LPS (1 µg/ml) and without those compounds (controls) for 2 h in RPMI 1640 medium enriched with 5% fetal bovine serum. ROS generation in PBMCs obtained from the incubated samples was estimated by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. The impact of E407a, LPS and their mixture on leukocyte cell membranes was evaluated spectrofluorimetrically using the fluorescent probe 2-(2¢-hydroxy-phenyl)-5-phenyl-1,3-oxazole.\\n\\n\\nResults: Expectedly, incubation with LPS induced ROS generation in PBMCs and decreased the lipid order of cell membranes in leukocytes. E407a alone was found to alter neither ROS production in PBMCs, nor membrane lipid order in leukocytes. Semi-refined carrageenan partially reduced LPS-mediated ROS overproduction in PBMCs and cell membrane alterations in leukocytes.\\nConclusion: E407a attenuates LPS-induced alterations of redox homeostasis in rat PBMCs and LPS-mediated modifications of cell membrane lipid order in leukocytes.\\n\",\"PeriodicalId\":16959,\"journal\":{\"name\":\"Journal of Pharmacy and Nutrition Sciences\",\"volume\":\"57 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pharmacy and Nutrition Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29169/1927-5951.2021.11.20\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacy and Nutrition Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29169/1927-5951.2021.11.20","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0

摘要

目的:探讨半精制角叉菜胶(E407a)对脂多糖(LPS)诱导的外周血单核细胞活性氧(ROS)生成及脂多糖介导的白细胞细胞膜改变的影响。方法:取8只完整大鼠血样,分别用E407a (10 mg/ml)、E407a (50 mg/ml)、LPS(1µg/ml)、E407a (10 mg/ml) + LPS(1µg/ml)、E407a (50 mg/ml) + LPS(1µg/ml)和不加这些化合物(对照组)在含有5%胎牛血清的RPMI 1640培养基中培养2 h。通过2',7'-二氯双氢荧光素(H2DCFDA)染色,流式细胞术估计培养样品中pbmc的ROS生成。采用荧光探针2-(2¢-羟基苯基)-5-苯基-1,3-恶唑,测定了E407a、LPS及其混合物对白细胞细胞膜的影响。结果:正如预期的那样,LPS诱导pbmc产生ROS,并降低白细胞细胞膜脂质顺序。单独E407a既不能改变PBMCs中ROS的产生,也不能改变白细胞的膜脂秩序。半精制卡拉胶部分减少了脂多糖介导的pbmc中ROS的过量产生和白细胞的细胞膜改变。结论:E407a可减弱lps诱导的大鼠pbmc氧化还原稳态的改变和lps介导的白细胞细胞膜脂质秩序的改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Semi-Refined Carrageenan Attenuates Lipopolysaccharide-Induced Reactive Oxygen Species Production in Peripheral Blood Mononuclear Cells and Cell Membrane Alterations in Leukocytes
Aim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes. Methods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml), LPS (1 µg/ml), E407a (10 mg/ml) + LPS (1 µg/ml), E407a (50 mg/ml) + LPS (1 µg/ml) and without those compounds (controls) for 2 h in RPMI 1640 medium enriched with 5% fetal bovine serum. ROS generation in PBMCs obtained from the incubated samples was estimated by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. The impact of E407a, LPS and their mixture on leukocyte cell membranes was evaluated spectrofluorimetrically using the fluorescent probe 2-(2¢-hydroxy-phenyl)-5-phenyl-1,3-oxazole. Results: Expectedly, incubation with LPS induced ROS generation in PBMCs and decreased the lipid order of cell membranes in leukocytes. E407a alone was found to alter neither ROS production in PBMCs, nor membrane lipid order in leukocytes. Semi-refined carrageenan partially reduced LPS-mediated ROS overproduction in PBMCs and cell membrane alterations in leukocytes. Conclusion: E407a attenuates LPS-induced alterations of redox homeostasis in rat PBMCs and LPS-mediated modifications of cell membrane lipid order in leukocytes.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Pharmacy and Nutrition Sciences
Journal of Pharmacy and Nutrition Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
自引率
0.00%
发文量
0
期刊最新文献
Assessing the Efficacy of Natural Pet Products in Protecting Gastric Cells and Reducing Cytotoxicity under Hyperacidity Conditions: An In Vitro Study Antioxidant Effect of a Combination of S-Acetyl-L-Glutathione, Vitamin E, Silybum Marianumon Hepatic Cells under Oxidative Stress: An In Vitro Study Protective Effect of Curcuminoids Consumption on Cadmium-Induced Testicular Injury in Albino Rats Clinical Trial: Management of Post-Haemorrhoidectomy Wound Healing by Bergamot Flavonoid-Based Gel and Sodium Hyaluronate: An Observational, Multicentric Trial Acid Suppressant Activity of Feed Dietary Supplements for Dogs
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1