The Modulatory Effects of Aqueous Extract of the Plant Biebersteinia Multifida on the Gastric Acid Level and Intestinal Cytokines in Ulcerative Colitis Model

BACKGROUND AND OBJECTIVE: Inflammatory bowel disease is a chronic disease of the gastrointestinal tract with unknown etiology, which includes crohn's disease and ulcerative colitis (UC). Because an effective treatment has not been found so far, the present study was designed to investigate the effects of aqueous extracts of Biebersteinia multifida plant on inflammatory changes of the colon following acetic acidinduced ulcerative colitis in male rats. METHODS: In this experimental study, 32 male wistar rats weighing 200-250 g were used in vivo. Animals were randomly assigned into four groups (each group consisting of 8 rats): 1Intact; 2Acetic acid-induced ulcerative colitis; 3Biebersteinia multifida aqueous extract (200 mg /Kg); 4Sulfasalazine treatment group (500 Mg /kg). In each group, the amount of gastric acid and intestinal TNF-α and IL-10 cytokines were measured at 8 days after of ulcerative colitis induction. FINDINGS: The concentration of gastric acid in the induction group of colitis was 2/4533 ± 0.95 mEq/15 min, which in the treatment group with Biebersteinia multifida extract decreased the level of gastric acid to 0.4400 ± 0.17 (p = 0.000). Also, TNF-α changes in the colitis and extract group were 279.68±42.71 and 160.35±28.79, respectively, which was significant (p=0.000). The concentration of IL-10 in the colitis group was 349.75±31.89, which increased to 353.75±66.46 after injection of the extract (p=0.015). CONCLUSION: The results showed that aqueous extract of Biebersteinia multifida in a dose of 200 mg/kg has an effect on colon inflammation and it can be improved.