利用鼻咽和肺泡灌洗液样本检测父子新型冠状病毒肺炎的不同RT-PCR诊断特征

Xingqiao Tao, Guohang Yuan, Shanshan Rao, Dan Li, Yaqin Liu, Xiangyan Zhang, Hong Yu, Yongqun He, Xianwei Ye
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引用次数: 1

摘要

实时RT-PCR检测病毒核酸是目前国内外临床确诊新冠肺炎的主要方法。然而,其敏感性尚不清楚。在此,我们报告中国西南部贵州省一个家庭中的两例病例。父亲曾在武汉长期居住。令人惊讶的是,尽管儿子通过鼻咽拭子标本和rRT-PCR方法被诊断为阳性,但父亲连续多次被诊断为阴性。仅在使用肺泡灌洗液样本后,父亲的rRT-PCR诊断才转为阳性。他们的CT诊断和临床症状与他们的rRT-PCR诊断结果不完全一致。讨论了潜在的机制及其对临床实践的影响。
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Distinct RT-PCR diagnosis profiles of father and son patients of COVID-19 using nasopharyngeal and alveolar lavage fluid samples
The detection of viral nucleic acid by real time RT-PCR is the main confirmative diagnostic method for COVID-19 in clinical practice in China and worldwide. However, its sensitivity is unclear. Here we report two cases in a family in Guizhou, southwestern China. The father had a history of long stay in Wuhan. Surprisingly, although the son was diagnosed positive using the nasopharyngeal swab specimen and the rRT-PCR method, the father was diagnosed negative continuously for multiple times. Only after the alveolar lavage fluid sample was used, the father’s rRT-PCR diagnosis turned positive. Their CT diagnosis and clinical symptoms did not completely align with their rRT-PCR diagnostic results. The underlying mechanisms and their implications to clinical practice are discussed.
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