水银花提取物的抗氧化和抗炎活性

K. Kim, Sook-He Kim
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引用次数: 1

摘要

本研究对水银花提取物的抗氧化和抗炎作用进行了初步研究。抗氧化实验包括多酚浓度测定、类黄酮浓度测定、DPPH实验、ABTS实验、NO实验和FRAP实验。多酚含量为54.85±2.79 mg/g。总黄酮含量为18.70±5.26 mg/g。DPPH试验显示抗坏血酸含量为3.950 mg /g, ABTS试验显示抗坏血酸含量为7.418 mg /g, NO试验显示抗坏血酸含量为6.056 mg /g。在FRAP中,1 mg艾灸提取物可使抗坏血酸减少3.633±0.280 μg。同时,细胞实验显示其对LPS诱导的炎症具有细胞毒性和抗炎作用。在细胞毒性实验中,水合银提取物在所有浓度下的细胞存活率均大于80%,在100 μg/mL浓度下的炎症抑制率为26.94±0.52%。这些结果表明,水银花提取物是一种抗炎的化妆品原料。
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Antioxidant and Anti-inflammatory activity of Silvetia siliquosa extract
In this study, the antioxidant and anti-inflammatory properties of Silvetia siliquosa extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 54.85 ± 2.79 mg/g was shown. Flavonoids showed 18.70 ± 5.26 mg/g. The DPPH experiment showed an antioxidant function of 3.950 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 7.418 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 6.056 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 3.633 ± 0.280 μg of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Silvetia siliquosa extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 26.94±0.52% at a concentration of 100 μg/mL. These results indicate that Silvetia siliquosa extract is available as an anti-inflammatory cosmetic material.
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