Nighat Nawaz, Irshad Ahmad, S. G. Patching, Iqbal, Hussain
{"title":"蒺藜提取物绿色合成金纳米颗粒及其对革兰氏阴性菌的抗菌活性","authors":"Nighat Nawaz, Irshad Ahmad, S. G. Patching, Iqbal, Hussain","doi":"10.47262/bl/7.2.20210617","DOIUrl":null,"url":null,"abstract":"Aqueous extract of the plant Tribulus terrestris was used to reduce chloroauric acid for synthesizing gold nanoparticles (AuNPs). In this green synthesis, the reaction proceeded to give a red/purple color that was monitored by UV-vis spectrophotometry, where the formed AuNPs had an absorption band with max of 550 nm. In terms of the highest absorbance at 550 nm, reaction conditions were optimized at a temperature of 75°C, at pH 7 and using a reaction time of 4 h. The integrity of the synthesized AuNPs was confirmed and their physical properties were characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM), which also showed evidence that plant metabolites contributed to capping or stabilization of the AuNPs. XRD spectra suggested a particle size of around 40 nm and SEM images revealed spherical and relatively uniform and disperse particles with a size of less than 0.1 m. In a broth microdilution assay, the AuNPs showed inhibitory effects against Gram-negative Escherichia coli, but not against Gram-positive Enterococcus faecalis. The AuNPs showed no hemagglutination activity or cytotoxic activity against human blood cells, which is important for them to be explored as therapeutic antibacterial agents.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"25 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria\",\"authors\":\"Nighat Nawaz, Irshad Ahmad, S. G. Patching, Iqbal, Hussain\",\"doi\":\"10.47262/bl/7.2.20210617\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aqueous extract of the plant Tribulus terrestris was used to reduce chloroauric acid for synthesizing gold nanoparticles (AuNPs). In this green synthesis, the reaction proceeded to give a red/purple color that was monitored by UV-vis spectrophotometry, where the formed AuNPs had an absorption band with max of 550 nm. In terms of the highest absorbance at 550 nm, reaction conditions were optimized at a temperature of 75°C, at pH 7 and using a reaction time of 4 h. The integrity of the synthesized AuNPs was confirmed and their physical properties were characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM), which also showed evidence that plant metabolites contributed to capping or stabilization of the AuNPs. XRD spectra suggested a particle size of around 40 nm and SEM images revealed spherical and relatively uniform and disperse particles with a size of less than 0.1 m. In a broth microdilution assay, the AuNPs showed inhibitory effects against Gram-negative Escherichia coli, but not against Gram-positive Enterococcus faecalis. The AuNPs showed no hemagglutination activity or cytotoxic activity against human blood cells, which is important for them to be explored as therapeutic antibacterial agents.\",\"PeriodicalId\":9154,\"journal\":{\"name\":\"Biomedical Letters\",\"volume\":\"25 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-09-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47262/bl/7.2.20210617\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47262/bl/7.2.20210617","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria
Aqueous extract of the plant Tribulus terrestris was used to reduce chloroauric acid for synthesizing gold nanoparticles (AuNPs). In this green synthesis, the reaction proceeded to give a red/purple color that was monitored by UV-vis spectrophotometry, where the formed AuNPs had an absorption band with max of 550 nm. In terms of the highest absorbance at 550 nm, reaction conditions were optimized at a temperature of 75°C, at pH 7 and using a reaction time of 4 h. The integrity of the synthesized AuNPs was confirmed and their physical properties were characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM), which also showed evidence that plant metabolites contributed to capping or stabilization of the AuNPs. XRD spectra suggested a particle size of around 40 nm and SEM images revealed spherical and relatively uniform and disperse particles with a size of less than 0.1 m. In a broth microdilution assay, the AuNPs showed inhibitory effects against Gram-negative Escherichia coli, but not against Gram-positive Enterococcus faecalis. The AuNPs showed no hemagglutination activity or cytotoxic activity against human blood cells, which is important for them to be explored as therapeutic antibacterial agents.