蒺藜提取物绿色合成金纳米颗粒及其对革兰氏阴性菌的抗菌活性

Nighat Nawaz, Irshad Ahmad, S. G. Patching, Iqbal, Hussain
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引用次数: 1

摘要

以蒺藜水提液为原料,还原氯金酸合成纳米金。在这个绿色合成中,反应继续产生红/紫色,通过紫外-可见分光光度法监测,其中形成的AuNPs具有最大550nm的吸收带。在温度为75℃、pH为7、反应时间为4 h的条件下,在550 nm处获得了最高吸光度。通过傅里叶变换红外(FTIR)光谱、x射线衍射(XRD)和扫描电镜(SEM)对合成的AuNPs的完整性进行了验证,并对其物理性质进行了表征,结果表明植物代谢物对AuNPs的覆盖或稳定起了作用。XRD光谱显示其粒径约为40 nm, SEM图像显示粒径小于0.1 m的球形且相对均匀分散的颗粒。在肉汤微量稀释试验中,AuNPs对革兰氏阴性大肠杆菌有抑制作用,但对革兰氏阳性粪肠球菌没有抑制作用。这些AuNPs对人血细胞无血凝活性或细胞毒活性,这对它们作为治疗性抗菌剂的探索具有重要意义。
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Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria
Aqueous extract of the plant Tribulus terrestris was used to reduce chloroauric acid for synthesizing gold nanoparticles (AuNPs). In this green synthesis, the reaction proceeded to give a red/purple color that was monitored by UV-vis spectrophotometry, where the formed AuNPs had an absorption band with max of 550 nm. In terms of the highest absorbance at 550 nm, reaction conditions were optimized at a temperature of 75°C, at pH 7 and using a reaction time of 4 h. The integrity of the synthesized AuNPs was confirmed and their physical properties were characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM), which also showed evidence that plant metabolites contributed to capping or stabilization of the AuNPs. XRD spectra suggested a particle size of around 40 nm and SEM images revealed spherical and relatively uniform and disperse particles with a size of less than 0.1 m. In a broth microdilution assay, the AuNPs showed inhibitory effects against Gram-negative Escherichia coli, but not against Gram-positive Enterococcus faecalis. The AuNPs showed no hemagglutination activity or cytotoxic activity against human blood cells, which is important for them to be explored as therapeutic antibacterial agents.
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