猪硫氧还蛋白cDNA序列

GraceHu Yu, Jiang-ying Xu, Lin Xu, Kin-Sing Lee
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引用次数: 5

摘要

人类、猕猴、羊、牛、马、鼠等几种哺乳动物硫氧还蛋白cDNA序列已在Genbank数据库中登记;但猪的情况尚不清楚。在这篇通讯中,我们报告了用RT-PCR方法测定猪硫氧还蛋白的全长cDNA序列。我们还比较了不同哺乳动物硫氧还蛋白的蛋白序列。猪与其他哺乳动物氨基酸序列的多序列比对表明,这些序列具有高度的保守性。猪和牛硫氧还蛋白的氨基酸序列只有一个不同。
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cDNA Sequence of Porcine Thioredoxin
Several mammalian thioredoxin cDNA sequences, namely that of human, macaca, ovine, bovine, equine and murine have been already registered in the Genbank database; but that of porcine is still not known. In this communication, we report the full-length cDNA sequence of porcine thioredoxin as determined by RT-PCR method. We also compared the protein sequence of thioredoxin from various mammals. Multialignment of the amino acid sequences between porcine and other mammalian species revealed that the sequences are highly conserved. Only one difference exists between the amino acid sequences of porcine and bovine thioredoxin.
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