Aleshkin Av, O. Borisova, Gadua Nt, S. Bochkareva, V. A. Chernova, Trebunskikh Ip, B. A. Efimov, L. Kafarskaya, S. Afanasiev, Aleshkin Va, M. Afanasiev, A. Borisova, A. Karaulov
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引用次数: 0
摘要
的目标。评价溴化氢患者腋窝皮肤微生物病的微生物组成。材料和方法。23名患者(11 - 17岁)在国家外科医疗中心皮罗戈夫中心接受了检查。使用生化检测系统BioMerieux VITEK MS MALDI-TOF(«BioMerieux»,法国)进行鉴定,并对16SrRNA基因进行测序,随后与EMBL/NCBI并并。腋臭患者皮肤中、高度微生物群播种率分别为52.2%和43.5%。共检出杆状杆菌、葡萄球菌、莫拉菌、微球菌、念珠菌和芽孢杆菌5属137株,其中以coiynehacium属(8种)和葡萄球菌属(5种)为主(89.1%)。硬脂结核杆菌在棒状杆菌和人型葡萄球菌中占主导地位。结论。腋窝皮肤微生物性病变以棒状杆菌和葡萄球菌为主的微生物群为主,占82.6%。
Aim. Evaluate the composition of microorganisms of skin microbiocenosis of axilla in brom-hidrosis patients. Materials and methods. 23 patients were examined (11 - 17 years) under the observation at Pirogov CCDC of the National Medical-Surgery Centre. Identification was carried out using biochemical test-systems BioMerieux VITEK MS MALDI-TOF («bioMerieux», France) and 16SrRNA genesequencing with consequent juxtaposition with EMBL/NCBI. Medium and high degree of skin seeding with microbiota was present in most of the patients with bromhidrosis (52.2 and 43.5%). 137 strains belonging to 5 genera of microorganisms were identified - Corynebacterium, Staphylococcus, Moraxella, Micrococcus, Candida and Bacillus spp. Coiynehacte-rium genus strains (8 species) and Staphylococcus genus (5 species) prevailed in microbiocenosis (89.1%). C. tuberculostearicum strains dominated among Corynebacterium, and S. hominis - Staphylococcus. Conclusion. In most of the cases (82.6%) in patients microbiocenosis of skin of axilla was presented by consortiums of microorganisms with prevalence of Corynebacterium and Staphylococcus microorganisms.