{"title":"从低温电子显微镜图像推断生物分子动力学的自由能景观","authors":"Mao Oide, M. Nakasako","doi":"10.5940/jcrsj.64.300","DOIUrl":null,"url":null,"abstract":"Cryo-electron microscopy(cryo-EM)is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble of the target biomolecule, cryo-EM is applicable to analyze the biomolecular dynamics such as metastable conformations and/or free-energy landscape in conformational changes. Here, we briefly introduce our method to deduce free-energy landscape of biomolecules from cryo-EM images and compare it with other existing methods.","PeriodicalId":74311,"journal":{"name":"Nihon Kessho Gakkai shi","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Deducing Free-Energy Landscape of Biomolecular Dynamics from Cryo-Electron Microscopy Images\",\"authors\":\"Mao Oide, M. Nakasako\",\"doi\":\"10.5940/jcrsj.64.300\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cryo-electron microscopy(cryo-EM)is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble of the target biomolecule, cryo-EM is applicable to analyze the biomolecular dynamics such as metastable conformations and/or free-energy landscape in conformational changes. Here, we briefly introduce our method to deduce free-energy landscape of biomolecules from cryo-EM images and compare it with other existing methods.\",\"PeriodicalId\":74311,\"journal\":{\"name\":\"Nihon Kessho Gakkai shi\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nihon Kessho Gakkai shi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5940/jcrsj.64.300\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nihon Kessho Gakkai shi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5940/jcrsj.64.300","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Deducing Free-Energy Landscape of Biomolecular Dynamics from Cryo-Electron Microscopy Images
Cryo-electron microscopy(cryo-EM)is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble of the target biomolecule, cryo-EM is applicable to analyze the biomolecular dynamics such as metastable conformations and/or free-energy landscape in conformational changes. Here, we briefly introduce our method to deduce free-energy landscape of biomolecules from cryo-EM images and compare it with other existing methods.