{"title":"用偶联法研究新鲜鸡肉中鼠伤寒沙门菌质粒毒力的自传性","authors":"S. Jabur, Aliaa A Hafz","doi":"10.24271/garmian.167","DOIUrl":null,"url":null,"abstract":"The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.","PeriodicalId":12283,"journal":{"name":"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study self-transmissibility of Salmonella Typhimurium plasmids virulence isolated from fresh chicken foods by conjugation\",\"authors\":\"S. Jabur, Aliaa A Hafz\",\"doi\":\"10.24271/garmian.167\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.\",\"PeriodicalId\":12283,\"journal\":{\"name\":\"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24271/garmian.167\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24271/garmian.167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Study self-transmissibility of Salmonella Typhimurium plasmids virulence isolated from fresh chicken foods by conjugation
The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.