IMPORTANCE OF ANALYTICALLY VERIFYING CHEMICAL TREATMENTS

Abstract Hydrogen peroxide is considered a low regμLatory priority compound by the U.S. Food and Drug Administration. It is used to control fungal infections on fish eggs. We studied the treatment profiles of hydrogen peroxide in Heath, McDonald egg jar, and Clark–Williamson incubators during treatments intended to deliver an effective regimen of at least 500 μL hydrogen peroxide/L (i.e., treatments of 500 and 1,000 μL/L) for 15 min. Hydrogen peroxide concentrations decreased with increasing distance from the influent water in both Heath and Clark–Williamson incubators. The top treatment tray (tray 2) of the Heath incubator received more than 90% of the intended regimen during the 500 μL/L treatment, whereas at 1,000 μL/L, all trays had hydrogen peroxide concentrations at or above 500 μL/L for 15 min. None of the compartments in the Clark–Williamson incubator received the intended therapeutic regimen when treated at 500 μL/L. The McDonald egg jar system distributed the intended concentration for the desig...