深层培养产胞外多糖和生物量的担子菌筛选

R. Maziero, V. Cavazzoni, V. Bononi
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引用次数: 76

摘要

对56株担子菌(包括从不同生态系统中分离的巴西本土真菌和食用菌)进行了体外培养和生物质生产的筛选。蘑菇(Agaricus sp.)和canarioudemansiella (Jungh.)培养7 d后,Hohn菌(ccb179)的胞外多糖产量最高,分别为6.01和3.54 g干w /l。产生物量最好的是裂藻(Schizophyllum commune);(CCB 473)为16.68 g干w /l,培养14 d。在多糖沉淀之前,将培养滤液冷冻,形成胶状部分。
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Screening of basidiomycetes for the production of exopolysaccharide and biomass in submerged culture
Fifty-six strains of Basidiomycetes, including native Brazilian fungi isolated from different ecosystems and edible mushrooms, were screened for production of exopolysaccharides and biomass in submerged culture. Agaricus sp. (CCB 280) and Oudemansiella canarii (Jungh.) Hohn (CCB 179) were the highest exopolysaccharide producers (6.01 and 3.54 g dry w./l respectively) after 7 days of incubation. The best producer of biomass was Schizophyllum commune Fr.:Fr. (CCB 473) with 16.68 g dry w./l in 14 days of incubation. When the culture filtrate was submitted to freezing prior to polysaccharide precipitation, a gelatinous fraction was formed.
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