标题伊拉克红曲霉新记录

Ghasaq Albrqawy, A.S.Saadon
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引用次数: 0

摘要

本研究在卡迪西耶大学生物系/理学院/真菌实验室进行,目的是分离和诊断从伊拉克卡迪西耶省进口干果(葡萄干)中分离的真菌的一些绝缘。利用分类键对分离株进行形态学和显微诊断,利用聚合酶链反应(PCR)对分离株进行外观和显微诊断,并利用ITS1和ITS4测定复合DNA产物的氮碱基序列(核苷酸序列)。利用BLAST与美国国家生物技术信息中心(NCBI)的数据进行比对,结果表明该分离株属于紫红曲霉(Monascus purpureus)型。通过比较本研究分离的紫红色分枝杆菌真菌的氮碱基序列,发现该分离株与此前在国家生物技术信息中心(NCBI)登记的许多紫红色分枝杆菌真菌分离株(包括中国的MT361825、MK359689、MW581230)和日本的AB477248)具有100%的相似性。
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New record of species Monascus purpureus in Iraq
This study was conducted in the Laboratory of Fungus in the Department of Biology / College of Science / University of Qadisiyah to isolate and diagnose some insulation from fungi isolated from imported dried fruits (raisins) in Qadisiyah province, Iraq. The isolations were diagnosed both morphologically and microscopically using the classification keys and to confirm the appearance and microscopic diagnosis diagnosed using polymerase chain reaction(PCR), And determine the sequence of nitrogen bases (Nucleotide sequence(of compound DNA products using ITS1 and ITS4. The results of the nucleotide sequence analysis of DNA (PCR product) compounding innate isolation and using BLAST to compare with data available at the U.S. National Center for Biotechnology Information (NCBI) have shown that this isolation belongs to the type Monascus purpureus. By comparing the sequence of nitrogen bases of isolated M. purpureus fungus in this study, it was found that there was a 100% similarity to many of the M. purpureus fungus isolates previously registered at the National Center for Biotechnology Information (NCBI), including those diagnosed in China (MT361825, MK359689, MW581230) and Japan (AB477248).
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