DPPH法测定桃金娘红、绿叶浸剂抗氧化性

Desy Muliana Wenas, Putrisa Anggun Meilani, Herdini Herdini
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摘要

红叶和绿叶含有黄酮类化合物、皂苷和单宁,它们具有抗氧化剂的潜力。目的是研究桃金子红芽红叶和绿叶浸剂的抗氧化活性。试验材料采用灌注法提取。采用1,1-二苯基-2-苦味酰肼(DPPH)法测定其抗氧化活性。采用紫外-可见分光光度法测定不同浓度的金缕梅叶浸提液对DPPH自由基浓度的影响。紫金桃叶浸提液采用不同浓度为20 ppm;10 ppm;5 ppm;2.5 ppm;1.25 ppm;0.625 ppm。5日制备维生素C溶液作为阳性对照;2.5;1.25;0625;0.3125 ppm。实验结果表明,红叶和绿叶粗提取物的IC50值分别为11130 ppm和10522 ppm。绿叶提取物的抗氧化活性高于红叶提取物。建议对桃金娘绿叶提取物的抗癌作用进行进一步研究。
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Antioxidant Test of Red and Green Colored-Leaves Infusions of Pucuk Merah (Syzygium myrtifolium Walp.) with DPPH method
Red and green leaves of the pucuk merah (Syzygium myrtifolium Walp.) contain flavonoids, saponins, and tannins which have the potential as antioxidants. The purpose is test out the antioxidant activity of red leaves and green leaves infusions of red shoots (S. myrtifolium). Test material is extracted using infusion method. The testing of antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The free radical concentration of DPPH was analyzed using UV-Vis spectrophotometer after addition of various concentrations of S. myrtifolium leaves infusion extracts. Various concentrations of S. myrtafolium leaves infusion extracts used were 20 ppm; 10 ppm; 5 ppm; 2.5 ppm; 1.25 ppm; 0.625 ppm. Vitamin C solutions as the positive control were prepared on 5; 2.5; 1.25; 0,625; 0.3125 ppm. The result of the experiment showed the thick red and green leaf extract has IC50 value of 11,130 ppm and 10,522 ppm. The antioxidant activity of green leaf extract is higher than the red leaf extract. Research on green leaves extract of S. myrtafolium is suggested for further research as anticancer.
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